FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences364864
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17540.480727065427118No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACAGTAAATGAAAAGAG14380.3941194527275917No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT13370.3664379056305911No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT10340.28339326433958956No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACAGTAAATGAAAAG10150.27818584458866863No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT8860.24283020522715315No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA8290.22720794597439045No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC8260.22638572180319244No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA8030.2200820031573408No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG8000.21925977898614277No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAAATC7870.21569680757761797No Hit
GTACGGGACCATGCAAAACTTGCACGACTCCTGCTCAAGGCAACTCTATG7600.20829679003683566No Hit
ATATAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC7520.2061041922469742No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT7520.2061041922469742No Hit
GTCTAAGGGCAGATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA7360.20171899666725138No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT7360.20171899666725138No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT7200.1973338010875285No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGTCACCCGTG6950.19048193299421154No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA6950.19048193299421154No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG6920.1896597088230135No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAGATATTTAGTGTGGGT6870.18828933520435012No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6490.17787449570250835No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6480.17760042097877565No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT6410.1756818979126469No Hit
CACTAAATATCTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC6350.17403744957025083No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA6290.17239300122785475No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC6170.16910410454306263No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA6030.1652670584108051No Hit
GTATACATGCATACAAAGGCATCAAGGCAGGATATCCACATTGTGTAAAT5850.1603337133836169No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC5790.15868926504122083No Hit
CTCCGAAAGAGACCAAATATTCAATTACAGTCTCTCTTCCAAAAGTAAGG5610.15375592001403263No Hit
GTCTTATATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT5600.15348184529029996No Hit
CTAAATATCTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAG5470.14991887388177513No Hit
ATTCTATCCTACCCACACTAAATATCTGCCCTTAGACAAAGGAATTAAAC5460.14964479915804244No Hit
GATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAAT5360.14690405192071568No Hit
ATTGATAGGTGTCAACAATTTGTGGGCCCTCTCACAGTAAATGAAAAGAG5050.13840773548500263No Hit
ATGTTAATACTAACATGGGTTTAAAGATCAGGCAACTATTGTGGTTTCAT5030.13785958603753729No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT4930.1351188388002105No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC4880.1337484651815471No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA4870.13347439045781442No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA4640.12717067181196282No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA4570.12525214874583407No Hit
ATTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTT4420.12114102788984389No Hit
TTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTC4320.1184002806525171No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT4250.11648175758638836No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTT4180.1145632345202596No Hit
CCATTTACACAATGTGGATATCCTGCCTTGATGCCTTTGTATGCATGTAT4150.11374101034906157No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG4120.11291878617786352No Hit
CAATTACAGTCTCTCTTCCAAAAGTAAGGCAAGATGTATGAAACCACAAT4070.11154841255920013No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT4030.11045211366426944No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA4010.10990396421680408No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAG3960.10853359059814069No Hit
CCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCT3960.10853359059814069No Hit
GTCTAGGAGATCTCTGACGGAAGGATAGAAGTCATGAGGCAAAAACGAGA3950.10825951587440799No Hit
ATCCTACCCACACTAAATATCTGCCCTTAGACAAAGGAATTAAACCTTAT3940.10798544115067532No Hit
CTCTCACAGTAAATGAAAAGAGAAGATTGAAATTAATTATGCCTGCTAGA3930.10771136642694264No Hit
GATCTGGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAGATATTTAGTG3900.1068891422557446No Hit
ATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGA3830.10497061918961585No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG3830.10497061918961585No Hit
ATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAG3810.1044224697421505No Hit
GTATAAAGGGACTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC3750.10277802139975444No Hit
CTCCTAGACACCGCCTCAGCTCTGTACCGAGAAGCCTTAGAGTCTCCTGA3750.10277802139975444No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG3700.10140764778109104No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)