FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482338
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN119912.4860160302526446No Hit
TCCATAGAGTGTGTAAATAATGTCGGGTTTGGAAGTAATGATTAACTAGA17720.36737723339235145No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAAC10540.21851896388010067No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT9460.19612802640472035No Hit
CCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCTGG8100.1679320310653525No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7790.1615050027159378No Hit
CTCTATGGAAGGCGGGTATCTTATATAAGAGAGAAACAACACATAGCGCC7370.15279741591995655No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAAAATCTAGTTAATCATTACT6950.1440898291239753No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAGACCACCGTG6920.14346785863854808No Hit
CACTAATATGGGCTTAAAGTTCAGACAACTTTTGTGGTTTCACATTTCTT6650.137870124269703No Hit
GTATATATGTGCCACATTTTCTTAATCCAGTCTATCATTGATGTACATTT6550.13579688931827888No Hit
GTGTAAATGGGGCAGCAAAACCCAAAAGACCCACAAAACGTTGACAGACT6360.1318577429105731No Hit
GTATTAAACCTTATTATCCAGAAAATCTAGTTAATCATTACTTCCAAACC6020.12480874407573113No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5670.11755242174574676No Hit
CATTTAAACCCTCATAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT5520.1144425693186106No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCGACCAATCGTCAATC5270.10925948194005033No Hit
CATTTACGCCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT5230.10843018795948069No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACG5130.10635695300805659No Hit
AATATACATTTACGCCAAGACATTATCAAAAAATGTGAACAATTTGTAGG5090.10552765902748695No Hit
CTCTTATATAAGATACCCGCCTTCCATAGAGTGTGTAAATAATGTCGGGT4970.10303977708577801No Hit
CCAAATACTCTGTGACCGTTTGTCTTCCAAAAGTGAGACAAGAAATGTGA4880.10117386562949633No Hit
GTGTAAATAATGTCGGGTTTGGAAGTAATGATTAACTAGATTTTCTGGAT4870.10096654213435391No Hit
CTCCGAAAGACACCAAATACTCTGTGACCGTTTGTCTTCCAAAAGTGAGA4830.10013724815378428No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)