FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483813
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18610.38465274806588495No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG17670.36522375380570593No Hit
CTTATATAGAATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT16710.3453813766889273No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG15010.3102438338779653No Hit
CTCTTATATAGAATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTGGT14250.2945352853271822No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA13840.28606093676689137No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT13320.27531298249530295No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12050.24906317110123125No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC11710.24203566253903885No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC11290.2333546225504482No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10790.22302005113545936No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGTT10600.21909291399776362No Hit
ATTCTATCCTAACCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC10560.21826614828456448No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA10080.2083449597261752No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG9890.2044178225884794No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC9840.20338436544698058No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC9360.19346317688859124No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTATTCCCTAAACTTCAT9250.1911895711772937No Hit
CCTATTAATAATATCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT9030.18664235975469862No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA9000.1860222854697993No Hit
GTCCCTTCGTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGG8750.18085499976230487No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA8720.18023492547740552No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA8460.17486094834161134No Hit
ATCCTAACCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT8440.17444756548501178No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT8250.170520428347316No Hit
TTTGGACGATCCAGCATCCAGGGATCTAGTAGTCAATTATGTTAATACTA7840.16204607978702515No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA7820.1616326969304256No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT7480.15460518836823317No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGGAACTCTATG7380.1525382740852354No Hit
GTCTAAGGGCAAATATTTAGTGTGGTTAGGATAGAATCTAGCAGGCATAA7330.15150481694373655No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC7320.15129812551543675No Hit
GGATATTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATG7210.1490245198041392No Hit
GTACAATCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACAATAGT7190.14861113694753963No Hit
GTGCTGGAGAGGATGTGGAGAAATAGGAACACTTTTACACTGTTGGTGGG7190.14861113694753963No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG7180.14840444551923987No Hit
ACAATCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACAATAGTTG7010.14489069123814366No Hit
TATTTAGTGTGGTTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT6880.14220370267024657No Hit
GCTCTATACCGAGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCA6840.14137693695704745No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6770.139930096958949No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT6600.13641634267785283No Hit
GTGCTGGCATCTGCTCAGTTTCTAGGGAGGCCGCAGGCAATTAACAATCA6590.13620965124955303No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT6430.1329025883967566No Hit
GTGCTGGGAGTGGCCCGGGGTGTTTTTCCATATTTGCTTTAGTGGAGTTC6380.13186913125525773No Hit
GGTTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA6370.13166243982695794No Hit
TAATTAATTTCAATCTTCTCTTTTCATTTACAGTGAGAGGGCCCACAAAT6320.13062898268545903No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT6280.12980221697225994No Hit
CCCTTCGTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCA6090.1258750798345642No Hit
GTATATACCCAAAGGACTATAAATCATGCTGCTATTAAGACACATGCACA6080.1256683884062644No Hit
CTACCGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG5940.12277470841006752No Hit
AGTCTGTACAACATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT5730.1184341884157722No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGATCACCCGTG5690.1176074227025731No Hit
CGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTAT5650.116780656989374No Hit
CCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCT5490.11347359413657757No Hit
GTTTCCCTCTTATATAGAATACCCGCCTTCCAAAGAGTATGTAAATAATG5460.11285351985167824No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGCAACTTTACTGGG5370.11099329699698023No Hit
GTACAACATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC5360.11078660556868046No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT5360.11078660556868046No Hit
ATGTACCCCATGAAGTTTAGGGAATAACCCCACCTTTTTGTTTTGTTAGG5330.11016653128378114No Hit
GGAAAATACAGACCCCTGACTCTGGGATCCTGCAGAGCTTGGTGGAAGGC5290.10933976557058202No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5290.10933976557058202No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT5260.10871969128568269No Hit
ATTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAA5230.10809961700078337No Hit
CTCCTAGACACCGCCTCAGCTCTATACCGAGAAGCCTTAGAGTCTCCTGA5230.10809961700078337No Hit
GCTTTATTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTT5200.10747954271588403No Hit
AGATATATGAAACCACAATAGTTGCCTGATCTTTAAACCCATGTTAGTAT5170.1068594684309847No Hit
GAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCT5150.10644608557438513No Hit
GTGTATATGTGCCACATTTTCTTAATCCAGTCTATCATTGTTGGACATTT5050.10437917129138738No Hit
TCCCATTACTGGGTATATACCCAAAGGACTATAAATCATGCTGCTATTAA4970.10272563986498916No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAATCTCTCTTCCAAAAGTAAGG4940.10210556558008983No Hit
CATTTAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT4920.10169218272349029No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG4890.10107210843859095No Hit
ATATAGAATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGA4880.10086541701029117No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG4850.10024534272539184No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)