FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483813
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN77431.600411729325173No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG15940.3294661367098445No Hit
CTTATATAGAATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT14340.2963955081818802No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG13520.2794468110612985No Hit
CTCTTATATAGAATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTGGT13280.27448621678210383No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA12580.2600178168011194No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT11800.24389588539373686No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC11270.23294123969384867No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10710.22136651970906113No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA10580.21867953114116404No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGTT10190.21061856543747276No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG10060.20793157686957564No Hit
ATTCTATCCTAACCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC9900.20462451401677922No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9380.1938765597451908No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC9130.18870927403769638No Hit
GTCCCTTCGTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGG9050.18705574261129815No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTATTCCCTAAACTTCAT8620.17816801119440775No Hit
CCTATTAATAATATCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT8580.17734124548120864No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC8480.17527433119821087No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA8050.16638659978132048No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA7950.1643196854983227No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA7920.16369961121342336No Hit
ATCCTAACCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT7610.1572921769361303No Hit
GGATATTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATG7310.15109143408713696No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC7270.15026466837393784No Hit
GTACAATCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACAATAGT7010.14489069123814366No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT6960.14385723409664478No Hit
TTTGGACGATCCAGCATCCAGGGATCTAGTAGTCAATTATGTTAATACTA6940.14344385124004522No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC6920.14303046838344569No Hit
GTGCTGGAGAGGATGTGGAGAAATAGGAACACTTTTACACTGTTGGTGGG6900.1426170855268461No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT6720.13889663981745012No Hit
GTCTAAGGGCAAATATTTAGTGTGGTTAGGATAGAATCTAGCAGGCATAA6700.13848325696085056No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG6690.1382765655325508No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGGAACTCTATG6680.13806987410425103No Hit
GTATATACCCAAAGGACTATAAATCATGCTGCTATTAAGACACATGCACA6670.13786318267595124No Hit
GGTTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA6530.13496950267975438No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT6510.1345561198231548No Hit
TATTTAGTGTGGTTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT6430.1329025883967566No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA6420.13269589696845682No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT6310.1304222912571593No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTC5780.1194676455572711No Hit
GTGCTGGCATCTGCTCAGTTTCTAGGGAGGCCGCAGGCAATTAACAATCA5780.1194676455572711No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5760.11905426270067154No Hit
ACAATCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACAATAGTTG5680.11740073127427333No Hit
GCTCTATACCGAGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCA5650.116780656989374No Hit
CCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCT5640.11657396556107422No Hit
TAATTAATTTCAATCTTCTCTTTTCATTTACAGTGAGAGGGCCCACAAAT5560.114920434134676No Hit
CTACCGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG5550.11471374270637623No Hit
GTTTCCCTCTTATATAGAATACCCGCCTTCCAAAGAGTATGTAAATAATG5500.11368028556487733No Hit
CCCTTCGTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCA5490.11347359413657757No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGATCACCCGTG5390.1114066798535798No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG5360.11078660556868046No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGCAACTTTACTGGG5340.1103732227120809No Hit
TGTCGGGACCGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCCG5340.1103732227120809No Hit
AGATATATGAAACCACAATAGTTGCCTGATCTTTAAACCCATGTTAGTAT5260.10871969128568269No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5250.10851299985738291No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT5220.10789292557248358No Hit
AGTCTGTACAACATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT5130.10603270271778559No Hit
CTCCTAGACACCGCCTCAGCTCTATACCGAGAAGCCTTAGAGTCTCCTGA5120.10582601128948581No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT5090.10520593700458647No Hit
GTGTATATGTGCCACATTTTCTTAATCCAGTCTATCATTGTTGGACATTT4960.10251894843668938No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT4860.10045203415369162No Hit
GAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCT4860.10045203415369162No Hit
ATGTACCCCATGAAGTTTAGGGAATAACCCCACCTTTTTGTTTTGTTAGG4850.10024534272539184No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)