FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250495
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA22020.8790594622647159No Hit
CTCTATGGAAGGCGGGTGTATTATATAAGAGAGAAACAACACATAGCGCC9250.36926884768159046No Hit
ATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA8540.34092496856224674No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACATAAACC8540.34092496856224674No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT8330.332541567695962No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8320.3321423581309008No Hit
CTCTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8260.32974710074053376No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA7590.30300005988143475No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG7160.28583404858380407No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT6860.2738577616319687No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT5630.2247549851294437No Hit
ATATAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC5350.2135771173077307No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT5260.20998423122218007No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5230.20878660252699657No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCTTTACATTTCAT5230.20878660252699657No Hit
GTGTAAAAGGGGCAGCGAAACCCAAAAGACCCACAATACGTTGACAGACT5200.20758897383181302No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG5110.20399608774626243No Hit
CTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT4990.19920557296552824No Hit
GAAAAGAAGACTGAACTTGATTATGCCTGCTAGGTTTTATCCAAAGGTTA4850.19361663905467175No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC4840.19321742948961057No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAAAAAGGCCTTGTAAGTTGGCGA4620.18443481905826464No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT4510.18004351384259168No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC4500.1796443042775305No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT4340.17325695123655163No Hit
GTATAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCCAATACCACATC4280.17086169384618455No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGACTTTATTCTTCTTCTA4220.1684664364558175No Hit
GTGTTGTTTCTCTCTTATATAATACACCCGCCTTCCATAGAGTGTGTAAA4140.16527275993532806No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTCTGT4080.16287750254496097No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3980.15888540689434918No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT3950.15768777819916566No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTGGGCAAGAATTGGTGGGCGTTC3910.15609093993892093No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT3810.15209884428830917No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG3790.15130042515818679No Hit
CTCATAAGGTGGGAAACTTTACGGGACTTTATTCTTCTTCTACTCCTGTC3760.15010279646300326No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCGAAACCCAAAAGACCCACAAT3680.14690911994251382No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3660.14611070081239147No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAGTCGTCAGTC3650.1457114912473303No Hit
CTCTATAACCGTTTCTCTTCCAAAAGTAAGACAAGAAATGTGAAACCACA3600.14371544342202439No Hit
GTACAGCATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC3590.1433162338569632No Hit
CTTCTACTCCTGTCTTTAACCCTAATTGGAAAACACCCTCTTTTCCTAAT3580.14291702429190203No Hit
CTTTAGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA3500.1397233477714126No Hit
ACTTTATTCTTCTTCTACTCCTGTCTTTAACCCTAATTGGAAAACACCCT3390.13533204255573963No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT3370.13453362342561728No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC3340.13333599473043375No Hit
CTCACAGTCAATGAGAAAAGAAGACTGAACTTGATTATGCCTGCTAGGTT3330.13293678516537255No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCA3310.1321383660352502No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT3300.13173915647018902No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCATTATATGCATGTATT3290.13133994690512785No Hit
CTTTAACCCTAATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC3290.13133994690512785No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGACTTTATTC3260.13014231820994432No Hit
TCCTCAGGCCATGCAGTGGAACTCCACGACCTTCCACCAAACTCTGCAAG3200.12774706081957724No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG3160.1261502225593325No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT3150.12575101299427133No Hit
CCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGAC3100.12375496516896543No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT3100.12375496516896543No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3050.12175891734365957No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT3050.12175891734365957No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA3040.12135970777859836No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG2990.1193636599532925No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATTAGGGTTAAAGA2960.11816603125810894No Hit
CATGCATATAATGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC2960.11816603125810894No Hit
TAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT2920.11656919299786422No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC2920.11656919299786422No Hit
GAATAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA2860.11417393560749715No Hit
GAACTTGATTATGCCTGCTAGGTTTTATCCAAAGGTTACCAAATATTTGC2840.1133755164773748No Hit
GAATATGGTGACCCACAAAATGAGGCGCTATGTGTTGTTTCTCTCTTATA2820.11257709734725245No Hit
GAATACATGCATATAATGGCATTAAAGCAGGATAACCACATTGTGTAAAA2790.11137946865206891No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA2790.11137946865206891No Hit
TCCACAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA2760.11018183995688538No Hit
GCTTTAGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG2760.11018183995688538No Hit
GTCAATGAGAAAAGAAGACTGAACTTGATTATGCCTGCTAGGTTTTATCC2750.10978263039182418No Hit
GGGTTATTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGTTCAT2740.109383420826763No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT2730.10898421126170182No Hit
ATGTGTTGTTTCTCTCTTATATAATACACCCGCCTTCCATAGAGTGTGTA2710.10818579213157947No Hit
GACATAGCCCATGAAATGTAAAGAATAACCCCATCTTTTTGTTTTGTTAG2670.10658895387133475No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCCTCGGACGGAAATTGCACC2650.1057905347412124No Hit
ATATTAGTGTTAACATAACTGACTACAAGGTCCCTGGATGTTGGATCTTC2650.1057905347412124No Hit
CCTAATACATAAACCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTC2640.10539132517615121No Hit
TAACCACATTGTGTAAAAGGGGCAGCGAAACCCAAAAGACCCACAATACG2640.10539132517615121No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTATACC2570.10259685822072297No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA2530.10100001996047826No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATAA2510.1002016008303559No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)