FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250495
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA22280.8894389109563064No Hit
CTCTATGGAAGGCGGGTGTATTATATAAGAGAGAAACAACACATAGCGCC9870.39401984071538354No Hit
CTCTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT9220.3680712189864069No Hit
ATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA9180.3664743807261622No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG8990.35888939898999983No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT8330.332541567695962No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACATAAACC8190.3269526337851055No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7960.31777081378869837No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA7900.3153755563983313No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT6690.26707119902592863No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT6110.24391704425238028No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5800.23154154773548374No Hit
GTGTAAAAGGGGCAGCGAAACCCAAAAGACCCACAATACGTTGACAGACT5460.21796842252340365No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC5380.2147747460029142No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG5340.2131779077426695No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT5300.2115810694824248No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCTTTACATTTCAT5060.20200003992095653No Hit
CTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT4980.1988063634004671No Hit
CTTTAGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA4900.19561268687997763No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC4500.1796443042775305No Hit
CTCTATAACCGTTTCTCTTCCAAAAGTAAGACAAGAAATGTGAAACCACA4390.17525299906185754No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT4370.17445457993173516No Hit
GTATAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCCAATACCACATC4320.17245853210642925No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGACTTTATTCTTCTTCTA4270.17046248428112337No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG4140.16527275993532806No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT4130.16487355037026688No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAAAAAGGCCTTGTAAGTTGGCGA4110.16407513124014453No Hit
GTGTTGTTTCTCTCTTATATAATACACCCGCCTTCCATAGAGTGTGTAAA4100.16367592167508335No Hit
CTCATAAGGTGGGAAACTTTACGGGACTTTATTCTTCTTCTACTCCTGTC3980.15888540689434918No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3980.15888540689434918No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT3900.15569173037385975No Hit
CTTCTACTCCTGTCTTTAACCCTAATTGGAAAACACCCTCTTTTCCTAAT3890.15529252080879857No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCGAAACCCAAAAGACCCACAAT3880.1548933112437374No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCATTATATGCATGTATT3870.15449410167867622No Hit
TCCTCAGGCCATGCAGTGGAACTCCACGACCTTCCACCAAACTCTGCAAG3840.1532964729834927No Hit
GAAAAGAAGACTGAACTTGATTATGCCTGCTAGGTTTTATCCAAAGGTTA3760.15010279646300326No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT3700.14770753907263617No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG3650.1457114912473303No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTCTGT3640.14531228168226912No Hit
ATATAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC3600.14371544342202439No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3570.14251781472684086No Hit
GTACAGCATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC3530.14092097646659615No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAGTCGTCAGTC3520.14052176690153498No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA3510.14012255733647377No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT3390.13533204255573963No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGACTTTATTC3300.13173915647018902No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC3240.12934389907982197No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT3220.1285454799496996No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCA3220.1285454799496996No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCCTCGGACGGAAATTGCACC3170.1265494321243937No Hit
CTTTAACCCTAATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC3160.1261502225593325No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTATACC3160.1261502225593325No Hit
GCTTTAGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG3140.12535180342921015No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC3080.1229565460388431No Hit
CCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGAC3010.12016207908341484No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATTAGGGTTAAAGA2980.1189644503882313No Hit
TAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT2970.11856524082317012No Hit
CTCACAGTCAATGAGAAAAGAAGACTGAACTTGATTATGCCTGCTAGGTT2960.11816603125810894No Hit
ACTTTATTCTTCTTCTACTCCTGTCTTTAACCCTAATTGGAAAACACCCT2910.11616998343280305No Hit
GAATATGGTGACCCACAAAATGAGGCGCTATGTGTTGTTTCTCTCTTATA2880.11497235473761952No Hit
ATGTGTTGTTTCTCTCTTATATAATACACCCGCCTTCCATAGAGTGTGTA2870.11457314517255833No Hit
GAACTTGATTATGCCTGCTAGGTTTTATCCAAAGGTTACCAAATATTTGC2850.11377472604243598No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA2830.11297630691231363No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTGGGCAAGAATTGGTGGGCGTTC2800.11177867821713008No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAA2750.10978263039182418No Hit
ATATTAGTGTTAACATAACTGACTACAAGGTCCCTGGATGTTGGATCTTC2700.10778658256651831No Hit
GTATTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACC2660.10618974430627358No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG2640.10539132517615121No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT2630.10499211561109004No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT2620.10459290604602886No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT2620.10459290604602886No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA2610.10419369648096768No Hit
GAATACATGCATATAATGGCATTAAAGCAGGATAACCACATTGTGTAAAA2590.10339527735084533No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA2590.10339527735084533No Hit
ATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGACTTTAT2580.10299606778578414No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT2580.10299606778578414No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)