FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494582
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN54201.0958749004209616No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG24250.4903130320149136No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATCATGCCTGCTAGG22320.4512901803947576No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG20730.4191418207698622No Hit
ATCCTACCCTTACCAAATATTTGCCCTTAGATAAAGGCATAAAACCTTAT14250.28812209097783587No Hit
GGATAATAAGGTTTTATGCCTTTATCTAAGGGCAAATATTTGGTAAGGGT12950.26183726864301576No Hit
GGGTAGGATAAAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCA11800.23858531042375178No Hit
CTATATGTTCAGGATAATAAGGTTTTATGCCTTTATCTAAGGGCAAATAT9880.19976464974463284No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA9580.1936989215135205No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT9450.1910704392800385No Hit
GTTCAGGATAATAAGGTTTTATGCCTTTATCTAAGGGCAAATATTTGGTA9430.19066605739796436No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9050.18298280163855538No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTTCCTATTA8790.17772583717159135No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTCTT8750.17691707340744306No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8620.17428859117396106No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTGAGA8560.17307544552773857No Hit
GGATAAAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCATTAAC8490.17166010894047903No Hit
CTCTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT8340.16862724482492286No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA8340.16862724482492286No Hit
ACTATATGTTCAGGATAATAAGGTTTTATGCCTTTATCTAAGGGCAAATA8340.16862724482492286No Hit
CCTCAAGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8260.16700971729662623No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG8180.16539218976832962No Hit
CCCTTTACACAATGTGGGTACCCAGCATTAATGCCTTTGTATGCATGTAT8120.16417904412210715No Hit
CAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGA7920.1601352253013656No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7840.15851769777306898No Hit
GTTTTATCCTACCCTTACCAAATATTTGCCCTTAGATAAAGGCATAAAAC7810.15791112494995774No Hit
CTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7670.15508045177543864No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTCTTGTGGTTTCACATTTCC7470.1510366329546971No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATCATGCCTGCTAGGTT7380.1492169144853634No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA7320.14800376883914093No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGGAAAACCCC7230.14618405036980722No Hit
TCTCAGGAGTTACTCTCTTTTTTGCCTACTGACTTCTATCCTTCTATTCG7120.14395995001839937No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGAAAACTCCCTCTTTTCCTAAT7100.1435555681363252No Hit
TTGACATACTTTCCAATCAATAGGTCTATTAATAGGAAGTTTCCGAAAAC7080.14315118625425105No Hit
GGTCTATTAATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAAT6960.14072489496180612No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA6780.13708545802313873No Hit
CAATAGGTCTATTAATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGT6740.13627669425899042No Hit
CTTTACACCGCTATAACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA6740.13627669425899042No Hit
GTTTGGAAGTAATGATTAACTATATGTTCAGGATAATAAGGTTTTATGCC6420.12980658414580393No Hit
GTCTATTAATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATA6340.12818905661750732No Hit
ATCTAAGGGCAAATATTTGGTAAGGGTAGGATAAAACCTAGCAGGCATGA6250.1263693381481736No Hit
GTTTTATGCCTTTATCTAAGGGCAAATATTTGGTAAGGGTAGGATAAAAC6250.1263693381481736No Hit
ATTTACATACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACT6170.12475181061987697No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGACTA6150.12434742873780283No Hit
ATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAA6110.12353866497365451No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTGAGACAGGAAATGTGAAACCACA5810.1174729367425422No Hit
GTTAATATGGGCCTAAAAATCAGACAACTCTTGTGGTTTCACATTTCCTG5720.1156532182732085No Hit
ATGTAATTGGAAGTTGGGGTACCTTGCCACAGGAACATATTGTACAAAAA5660.11444007262698602No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5650.11423788168594895No Hit
GAAAAAAGGAGATTAAAATTAATCATGCCTGCTAGGTTTTATCCTACCCT5570.11262035415765233No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC5540.11201378133454108No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA5470.11059844474728155No Hit
AAATTAATCATGCCTGCTAGGTTTTATCCTACCCTTACCAAATATTTGCC5360.10837434439587368No Hit
GATTAACTATATGTTCAGGATAATAAGGTTTTATGCCTTTATCTAAGGGC5280.10675681686757707No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT5250.10615024404446584No Hit
ATTAATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT5240.10594805310342875No Hit
ACCCAGGATCCAGGGAATTAGTAGTAAGCTATGTCAATGTTAATATGGGC5220.1055436712213546No Hit
TCCTCATATTCATTTGAAGGAGGATATTATCAATAGATGTCAACAATATG5170.1045327165161692No Hit
CCTTTATACCGCTATAACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5000.1010954705185389No Hit
TTCCTCTACTGTACCTGTCTTTAATCCTGACTGGAAAACTCCCTCTTTTC4960.10028670675439058No Hit
CCTTTATCTAAGGGCAAATATTTGGTAAGGGTAGGATAAAACCTAGCAGG4960.10028670675439058No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)