FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494582
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN104952.1219939261841314No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG28290.571998172193893No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATCATGCCTGCTAGG20760.4197483935929735No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG18960.38335402420629944No Hit
GGATAATAAGGTTTTATGCCTTTATCTAAGGGCAAATATTTGGTAAGGGT13040.2636569871123494No Hit
ATCCTACCCTTACCAAATATTTGCCCTTAGATAAAGGCATAAAACCTTAT11780.2381809285416776No Hit
GGGTAGGATAAAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCA11290.2282735724308608No Hit
GTTCAGGATAATAAGGTTTTATGCCTTTATCTAAGGGCAAATATTTGGTA9970.20158436821396652No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA9550.19309234869040928No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9540.19289015774937218No Hit
CTATATGTTCAGGATAATAAGGTTTTATGCCTTTATCTAAGGGCAAATAT9530.19268796680833514No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA9010.18217403787440706No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT8850.1789389828178138No Hit
CCTCAAGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8440.17064915423529364No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTCTT8390.16963819953010825No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTGAGA8050.16276370753484762No Hit
GTTTTATCCTACCCTTACCAAATATTTGCCCTTAGATAAAGGCATAAAAC8040.16256151659381052No Hit
ACTATATGTTCAGGATAATAAGGTTTTATGCCTTTATCTAAGGGCAAATA8010.1619549437706993No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG7910.15993303436032852No Hit
GGATAAAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCATTAAC7900.15973084341929145No Hit
CCCTTTACACAATGTGGGTACCCAGCATTAATGCCTTTGTATGCATGTAT7770.15710236118580942No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTTCCTATTA7760.15690017024477235No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7740.1564957883626982No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGGAAAACCCC7410.14982348730847464No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTCTTGTGGTTTCACATTTCC7280.1471950050749926No Hit
CTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7240.14638624131084432No Hit
GGTCTATTAATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAAT7120.14395995001839937No Hit
CTCTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT7090.14335337719528815No Hit
GTTTGGAAGTAATGATTAACTATATGTTCAGGATAATAAGGTTTTATGCC7080.14315118625425105No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7050.14254461343113983No Hit
TCTCAGGAGTTACTCTCTTTTTTGCCTACTGACTTCTATCCTTCTATTCG7050.14254461343113983No Hit
GTCTATTAATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATA6970.14092708590284322No Hit
CAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGA6770.13688326708210166No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA6730.13607450331795334No Hit
GTTTTATGCCTTTATCTAAGGGCAAATATTTGGTAAGGGTAGGATAAAAC6700.1354679304948421No Hit
ATCTAAGGGCAAATATTTGGTAAGGGTAGGATAAAACCTAGCAGGCATGA6670.13486135767173088No Hit
CTTTACACCGCTATAACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA6640.13425478484861963No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGAAAACTCCCTCTTTTCCTAAT6500.13142411167410056No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA6430.130008775086841No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGACTA6320.12778467473543315No Hit
CAATAGGTCTATTAATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGT6290.1271781019123219No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATCATGCCTGCTAGGTT6040.12212332838639496No Hit
TTGACATACTTTCCAATCAATAGGTCTATTAATAGGAAGTTTCCGAAAAC5870.11868608238876466No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5870.11868608238876466No Hit
GTTAATATGGGCCTAAAAATCAGACAACTCTTGTGGTTTCACATTTCCTG5840.11807950956565343No Hit
ACCCATCTCGGGGCCGTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCG5580.1128225450986894No Hit
ATTTACATACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACT5490.11100282662935569No Hit
ATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAA5470.11059844474728155No Hit
GATTAACTATATGTTCAGGATAATAAGGTTTTATGCCTTTATCTAAGGGC5410.10938529910105908No Hit
CCTTTATCTAAGGGCAAATATTTGGTAAGGGTAGGATAAAACCTAGCAGG5340.10796996251379953No Hit
ATGTAATTGGAAGTTGGGGTACCTTGCCACAGGAACATATTGTACAAAAA5190.10493709839824338No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC5160.10433052557513214No Hit
CCTTTATACCGCTATAACCAATTTTCTTTTGTCTTTGGGTATACATTTAA4960.10028670675439058No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)