FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429967
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20550.4779436561410527No Hit
GTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGATAATAAGGTTTA12190.2835101298471743No Hit
AATATACACTTACACCAAGACATTATCAAAAGATGTGAACAATTTGTAGG11790.274207090311582No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAACAAC11710.2723464824044636No Hit
CATTTAAATCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT11170.259787379031414No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT10960.2549032832752281No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC10870.2528100993797198No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10280.23908811606472127No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG10210.23746008414599262No Hit
CAAAAAATGTGAACAATTTGTAGGCCCACTCACAGTCAATGAGAAAAGAA9600.2232729488542144No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT9000.209318389550826No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT8860.2060623257133687No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT8620.20048050199201334No Hit
CACTTACACCAAGACATTATCAAAAGATGTGAACAATTTGTAGGCCCACT8450.19652671018938664No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8260.19210776640998029No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8200.19071231047964146No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT8120.188851702572523No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT7130.16582667972193213No Hit
TTACACACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC6980.16233803989608506No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG6930.161175159954136No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTAATTATGCCTGCTAGGTT6770.1574539441398991No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6690.15559333623278065No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6670.15512818425600103No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6620.153965304314052No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6580.15303500036049278No Hit
GAAAAGAAGACTGCAATTAATTATGCCTGCTAGGTTTTATCCAAATGTTA6410.14908120855786608No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA6370.14815090460430685No Hit
ATATAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGC6350.14768575262752723No Hit
CTTATATAATATACCTGCCTTCCACAGAGTGTGTAAATAATGTCTGGTTT6240.14512741675523935No Hit
CACCAAGACATTATCAAAAGATGTGAACAATTTGTAGGCCCACTCACAGT6070.14117362495261263No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA6070.14117362495261263No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6040.14047589698744323No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC5970.13884786506871458No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT5950.13838271309193495No Hit
CATCTTTTGATAATGTCTTGGTGTAAGTGTATATTAGGAAAAGAGGGTGT5800.13489407326608788No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCT5770.13419634530091845No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA5430.12628876169566502No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA5420.1260561857072752No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC5350.12442815378854656No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATGACAGAGAGTCCAA5350.12442815378854656No Hit
CTTATATAATATACCTGCCTTCCACAGAGTGTGTAAATAATGTCTAGTTT5310.12349784983498734No Hit
GTCTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATAC5210.12117208995108927No Hit
TGGGCATACATTTAAATCCTAACAAAACAAAAAGATGGGGTTACTCTTTA5170.12024178599753005No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5100.11861375407880141No Hit
ATTTATGCCTACAGCCTCCTAATACAAACAACTGTAATCTAATCTCCTCC5070.11791602611363197No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCTTTTCTCA5040.11721829814846255No Hit
GCACCGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT5040.11721829814846255No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5010.11652057018329313No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCTGTCATGTCAACGACCGACCT4980.11582284221812371No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4950.1151251142529543No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA4910.11419481029939507No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT4850.11279935436905623No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTTCCTGTC4850.11279935436905623No Hit
GTCAATGAGAAAAGAAGACTGCAATTAATTATGCCTGCTAGGTTTTATCC4830.11233420239227662No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCTCTAGATGTTGGATCTTC4820.11210162640388681No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT4780.11117132245032758No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA4750.11047359448515817No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA4720.10977586651998875No Hit
AGATCACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA4690.10907813855481933No Hit
GCAATTAATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGC4620.10745010663609068No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACACTTACACC4590.10675237867092127No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT4590.10675237867092127No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT4560.10605465070575185No Hit
GCTTAGATTGAATACATGCATACAAGGGCATTAAAGCAGGATAACCACAT4550.10582207471736205No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG4510.1048917707638028No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC4510.1048917707638028No Hit
TAATATACCTGCCTTCCACAGAGTGTGTAAATAATGTCTGGTTTGGAAGT4500.104659194775413No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC4450.10349631483346396No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC4420.10279858686829454No Hit
TCCACAGAGTGTGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTAGA4400.10233343489151492No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT4380.10186828291473532No Hit
CAAAAGATGTGAACAATTTGTAGGCCCACTCACAGTCAATGAGAAAAGAA4380.10186828291473532No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG4350.1011705549495659No Hit
TCCAACATCTAGAGACCTAGTAGTCAGTTATGTTAACACTAATATGGGCC4340.1009379789611761No Hit
CTCTTATATAATATACCTGCCTTCCACAGAGTGTGTAAATAATGTCTAGT4320.10047282698439647No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTCATGTCAACGACCGACCT4310.10024025099600667No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)