FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453551
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39350.8675981311914206No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG21810.4808720518750923No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT15750.34725973484790024No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTTCTTTTCTCG14960.32984162751267226No Hit
CTCTTACAGTTAACGAGAAAAGAAGATTAAAATTGATCATGCCTGCTAGG14720.32455005060070424No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG12100.2667836693117202No Hit
GTGTAAAGGGAGCGGCAAACCCCAAAAGACCCACAATTCTTTGACATACT10300.22709684247196016No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT10130.22334864215931616No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9500.20945825276540014No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTTCTTTTCTCGTTAAC8730.19248111017283612No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT8680.19137869831617613No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAAC8650.1907172512021801No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAT8640.19049676883084812No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT8550.18851242748886013No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8450.18630760377554012No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8260.18211843872023212No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA8080.17814975603625613No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT7670.1691099788116441No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7520.1658027432416641No Hit
ACCCATCTCGGGGCCGTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCG7250.15984971921570013No Hit
CCCTTAACTTCATGGGTTATGTAATTGGAAGTTGGGGTACCTTGCCACAG7050.1554400717890601No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA7020.1547786246750641No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6870.1514713891050841No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6840.1508099419910881No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC6830.1505894596197561No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATAACCCATGAA6800.1499280125057601No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA6800.1499280125057601No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC6780.1494870477630961No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT6730.1483846359064361No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTTCCTATAA6560.1446364355937921No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA6450.14221112950914008No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6040.1331713522845281No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5930.1307460461998761No Hit
GTTTGGAAGTAATGATTAACTATATGTTCAGGATAATAAGGTTTGATGCC5820.1283207401152241No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCATATTCATTTGAAAG5820.1283207401152241No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC5650.1245725398025801No Hit
CAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAGAAAAGAA5440.11994241000460808No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCT5360.11817855103395207No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA5260.11597372732063208No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT5240.11553276257796807No Hit
CAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGT5210.11487131546397207No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT5200.11465083309264007No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5190.11443035072130807No Hit
ATATAAGACCTTAGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC5150.11354842123598008No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5130.11310745649331608No Hit
ATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATGA5090.11222552700798807No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA5080.11200504463665607No Hit
CCTCCCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5050.11134359752266007No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5030.11090263277999607No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG4950.10913877380934008No Hit
ACCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCG4900.10803636195268007No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC4860.10715443246735207No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4750.10472912638270007No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAAT4710.10384719689737207No Hit
CCATCTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGGGA4700.10362671452604007No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT4680.10318574978337607No Hit
ATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAG4620.10186285555538407No Hit
CCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGG4600.10142189081272007No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTTTTGTC4580.10098092607005607No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)