FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436943
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47051.0767994910091248No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAACATAAGA15150.34672714747690203No Hit
GTAGTAGGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT13150.30095458675387865No Hit
GCCTACTACTAATTCCCTGGATGCAGGGTCTTCCAAATTACTTCCCACCC11160.2554108888344704No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10800.24717182790432618No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG10610.242823434635639No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGACTTTGGCCAC10460.2393904925814122No Hit
GTATGTAAATAATGCCTGGTTTGGAAGTAATGATTAACTGTATGTTCAGG9580.21925056586328193No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC9100.20826515128975634No Hit
CTCAAGAACAGTTTCTCTTCCAAACATAAGACAGGAAATGTGAAACCACA9030.2066631116644505No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9020.20643424886083542No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAA9020.20643424886083542No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA8580.19636428550177026No Hit
GGATACTAACATTGGGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA8520.19499110868007954No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG8120.18583659653547488No Hit
ACATAAGACAGGAAATGTGAAACCACAATAGTTGTCTGATTTTTAGGCCC8080.1849211453210144No Hit
ACATTGGGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACC8000.18309024289209347No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT7970.18240365448124812No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7770.1778263984089458No Hit
TATTAACATTGACATAGCCTACTACTAATTCCCTGGATGCAGGGTCTTCC7710.17645322158725507No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAATAGAAGGAAA7590.1737068679438737No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGTAGAAGAATAAAGCCCAG7570.17324914233664343No Hit
CTATTGTGGTTTCACATTTCCTGTCTTATGTTTGGAAGAGAAACTGTTCT7450.17050278869326205No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATGGACATTGA7260.16615439542457483No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAACATAAGACA7150.16363690458480853No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC7120.1629503161739632No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAG7030.16089055094142715No Hit
CCACAATAGTTGTCTGATTTTTAGGCCCATATTAACATTGACATAGCCTA6970.15951737411973643No Hit
GACATAGCCTACTACTAATTCCCTGGATGCAGGGTCTTCCAAATTACTTC6900.15791533449443063No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAGCACTAAGGCA6820.1560844320655097No Hit
GAATTAGTAGTAGGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA6750.15448239244020387No Hit
GCCTAGTCATCTCATGTTCATGTTCCACTGTTCAAGCCTCCAAGCTGTGC6740.15425352963658875No Hit
CTCTTACAGTTAACGAAAAAAGAAGATTAAAATTGATCATGCCTGCTAGG6740.15425352963658875No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC6560.15013399917151665No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT6320.14464129188475383No Hit
GTCCAAGGGATACTAACATTGGGGTTCCCGAGATTGAGATCTTCTGCGAC6310.14441242908113874No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT6310.14441242908113874No Hit
GGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTT6100.13960631020522127No Hit
ATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAATAGAAGGAAAGAAGT5990.137088819365455No Hit
ATCCTAACCGTACCAAATATTTACCCTTAGATAAAGGCATCAAACCTTAT5980.13685995656183989No Hit
ATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCAC5890.13480019132930382No Hit
GCCTAATCATCTCATGTTCATGTTCCACTGTTCAAGCCTCCAAGCTGTGC5890.13480019132930382No Hit
CTACTAATTCCCTGGATGCAGGGTCTTCCAAATTACTTCCCACCCAGGTG5830.13342701450761313No Hit
GAATAAAGCCCAGTAAAGTTTCCCACCTTGTGAGTCCAAGGGATACTAAC5720.13090952366784683No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGACTTTG5680.12999407245338637No Hit
GTATGGTGAGGTGTACAATGTTCCGGAGACTCTAAGGCCTCCCGATACAG5630.12884975843531077No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG5510.12610340479192939No Hit
TTGTACACCTCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTG5490.12564567918469913No Hit
GGGAATTAGTAGTAGGCTATGTCAATGTTAATATGGGCCTAAAAATCAGA5240.11992410909432123No Hit
CCTTAGTGCTGTATGGTGAGGTGTACAATGTTCCGGAGACTCTAAGGCCT5240.11992410909432123No Hit
GTCTTATGTTTGGAAGAGAAACTGTTCTTGAGTATTTGGTGTCTTTTGGA5230.1196952462907061No Hit
GTTAGTATCCCTTGGACTCACAAGGTGGGAAACTTTACTGGGCTTTATTC5220.11946638348709099No Hit
ATTCTGTGTTGGGGTGAGTTGATGACTTTGGCCACCTGGGTGGGAAGTAA5150.11786434386178517No Hit
AGATAGGGGCATTTGGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACT5100.11672002984370959No Hit
GTGTTGGGGTGAGTTGATGACTTTGGCCACCTGGGTGGGAAGTAATTTGG5030.11511799021840377No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATGGACATTGACCCGTAT4950.11328708778948285No Hit
GTTGATGACTTTGGCCACCTGGGTGGGAAGTAATTTGGAAGACCCTGCAT4920.11260049937863749No Hit
CATGTATACAAGCCAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4920.11260049937863749No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4910.11237163657502239No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG4910.11237163657502239No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCA4850.11099845975333167No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTTCTTTTTTCG4840.11076959694971657No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGTAAATATTTGGTACGGTT4800.10985414573525609No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4790.10962528293164096No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4760.1089386945207956No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC4710.10779438050272003No Hit
GAGCAGAGGCGGTGTCGAGGAGATCACGAATAGAAGGAAAGAAGTCAGAA4690.10733665489548981No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC4690.10733665489548981No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTGTGAGTCCAAGGGAT4640.1061923408774142No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTTCCTATAA4610.10550575246656886No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT4550.10413257564487817No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTCCCAATTACATATCCCATGAA4430.10138622200149676No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT4420.10115735919788164No Hit
ATATTAACATTGACATAGCCTACTACTAATTCCCTGGATGCAGGGTCTTC4400.10069963359065143No Hit
GTCATCAACTCACCCCAACACAGAATAGCTTGCCTTAGTGCTGTATGGTG4390.10047077078703628No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)