FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524644
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36900.7033340703410312No Hit
GAAACGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAAGGTTA23650.45078186351125715No Hit
CCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACAT22770.43400858486897775No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT19680.3751115041818833No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACA13960.2660851930070676No Hit
ATATAATACACCCGCCCTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA11590.2209117039363835No Hit
GAGTATCCCCATCTTTTCGTTTTGTTAGGGTTTAAATGTATACCCAGAGA10940.20852235039379086No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC10560.20127934370735204No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC10480.19975450019441757No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG10310.19651420772943176No Hit
TCTTTTGTCTCTGGGTATACATTTAAACCCTAACAAAACGAAAAGATGGG10270.19575178597296453No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCAT10240.19517996965561407No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10170.1938457315817964No Hit
CTCTAAGACCACTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9960.1898430173603434No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT9930.18927120104299297No Hit
CGTTAATATGGGCCTAAAGTTCAGACAACTATTGTGGTTTCACATTTCTT9680.1845060650650727No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGAGGTTATGGGTCAT9480.1806939562827365No Hit
GTGTAAAAGGAGCAGCAAAACCCAACAGACCCACGATACGTTGACAGACT9480.1806939562827365No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT9480.1806939562827365No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCATCTTTTC9000.17154489520512958No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTGAAACATTCTTTGATTTTCTGT8630.16449249395780757No Hit
CTCTATGGAGGGCGGGTGTATTATATCAGAGAGAAACAACACATAGCGCC8550.16296765044487307No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT8390.15991796341900413No Hit
GAGTAACCCCATCTTTTCGTTTTGTTAGGGTTTAAATGTATACCCAGAGA8350.1591555416625369No Hit
GTCCTAGGAATCCTGATGTGATGTTCTCCATGTTCAGCGCAGGGTCCCCA8340.1589649362234201No Hit
CTTCCAAAAGTGAGACAAGAAATGTGAAACCACAATAGTTGTCTGAACTT8240.15705888183225197No Hit
CTTTAACCCTCATTGGAAAACACCATCTTTTCCTAATATACATTTACACC8070.15381858936726617No Hit
CATCCAGAGACCTAGTAGTCGGTTATGTTAACGTTAATATGGGCCTAAAG7950.15153132409786446No Hit
GAACCACCGTGTGTCTTGGCCAAAATTCGCAGTGCCCAACCTCCAATCAC7580.14447892285054245No Hit
CTACATGTTCGGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATAT7570.14428831741142564No Hit
ATAATACACCCGCCCTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG7500.14295407933760798No Hit
CTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC7360.14028560318997263No Hit
GATTATGCCTGCTAGGTTTTATCCAAAGGTTACCAAATATTTGCCATTGG7270.13857015423792135No Hit
CGGTAGTCCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC7070.13475804545558512No Hit
CTTTATACCGCTGTTACCAATTTTCTTGTGTCTCTGGGTATACATTTAAA7020.13380501826000107No Hit
GAATCAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAAC6920.13189896386883296No Hit
CCTTTATACCGCTGTTACCAATTTTCTTGTGTCTCTGGGTATACATTTAA6880.13113654211236572No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6870.1309459366732489No Hit
GTCTTTGAAGTGTGCCTCAAGGTCGGTCGTTGGCAGAACAGAGAGTCCAA6750.12865867140384718No Hit
TCTAACAACAGTAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTG6640.12656201157356226No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG6630.12637140613444545No Hit
GTAGTCGGTTATGTTAACGTTAATATGGGCCTAAAGTTCAGACAACTATT6590.1256089843779782No Hit
GTGTTGTTTCTCTCTGATATAATACACCCGCCCTCCATAGAGTGTGTAAA6590.1256089843779782No Hit
GTTTTATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC6570.1252277734997446No Hit
GATATAATACACCCGCCCTCCATAGAGTGTGTAAATAATGTCTGGTTTGG6510.12408414086504371No Hit
CATTTAAACCCTAACAAAACGAAAAGATGGGGTTACTCTTTACATTTCAT6500.1238935354259269No Hit
CTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAA6450.12294050823034286No Hit
CCTCCGGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6270.11950961032624026No Hit
CTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAC6250.11912839944800664No Hit
GAAGATGAGGCATAGCAGCAGGATGAAGAGGAAGATGATAAAACGCCGCA6240.11893779400888983No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6190.11798476681330579No Hit
CTCACAGTCAATGAGAAACGAAGACTGCAATTGATTATGCCTGCTAGGTT6100.1162693178612545No Hit
CCCATGAATATGGTGACCCACAGAATGAGGCGCTATGTGTTGTTTCTCTC6070.11569750154390407No Hit
CTCTGATATAATACACCCGCCCTCCATAGAGTGTGTAAATAATGTCTGGT6060.11550689610478726No Hit
CAAGATCTACAGCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGAT6050.11531629066567042No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG6010.11455386890920319No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC6000.11436326347008638No Hit
GTTATTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCG5960.11360084171361914No Hit
GTCTTTAACCCTCATTGGAAAACACCATCTTTTCCTAATATACATTTACA5910.11264781451803509No Hit
GTCAATGAGAAACGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC5870.11188539276156785No Hit
GGATAAGGGTATTAAACCTTATTACCCCGAACATGTAGTTAATCATTACT5840.11131357644421742No Hit
CATCTAGAGACCTAGTAGTCGGTTATGTTAACGTTAATATGGGCCTAAAG5750.10959812749216612No Hit
CTCTAGATGTGTCATCTTGCAAATTACCACCCACCCAGGTGGCTAGAGTC5740.10940752205304931No Hit
GGGATACTCTTTACATTTCATGGGCTATGTCATTGGAGGTTATGGGTCAT5740.10940752205304931No Hit
GGATAAGGGTATTAAACCTTATTATCCCGAACATGTAGTTAATCATTACT5730.1092169166139325No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAAGCCC5710.10883570573569888No Hit
AGCCGGAGGAGTGCGAATCCACACTCCGAAAGATACCAAATACTCTAAGA5690.10845449485746524No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5620.10712025678364757No Hit
CTTTTTACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAA5470.10426117519689543No Hit
GTTCAGCTCACCATACCGCACTCAGGCAAGCAATTCTATGCTGGGGGGAA5440.10368935887954497No Hit
GTTCCTGTCTTTAACCCTCATTGGAAAACACCATCTTTTCCTAATATACA5390.10273633168396093No Hit
GTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGGTTAAAGA5340.10178330448837689No Hit
GGATAACCACATTGTGTAAAAGGAGCAGCAAAACCCAACAGACCCACGAT5340.10178330448837689No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCTTCCACCAACCGACAGTC5320.10140209361014325No Hit
GTAGCTCTTGTTCCCAAGAATATGGTGACCCACAGAATGAGGCGCTATGT5250.10006785553632559No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)