FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524644
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36750.7004749887542792No Hit
CCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACAT22170.42257225852196917No Hit
GAAACGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAAGGTTA20150.38406995982037345No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT18480.35223885148786604No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACA12230.23311045203985942No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC12100.23063258133134085No Hit
CTCTAAGACCACTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA11160.21271567005436068No Hit
ATATAATACACCCGCCCTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA11110.2117626428587766No Hit
GAGTATCCCCATCTTTTCGTTTTGTTAGGGTTTAAATGTATACCCAGAGA10910.20795053407644043No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG10470.19956389475530076No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGAGGTTATGGGTCAT10430.19880147299883352No Hit
GTGTAAAAGGAGCAGCAAAACCCAACAGACCCACGATACGTTGACAGACT10290.19613299685119814No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC10100.19251149350797875No Hit
TCTTTTGTCTCTGGGTATACATTTAAACCCTAACAAAACGAAAAGATGGG9730.18545909226065674No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9450.18012213996538606No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCAT9440.17993153452626925No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT9390.17897850733068518No Hit
CTTCCAAAAGTGAGACAAGAAATGTGAAACCACAATAGTTGTCTGAACTT8600.16392067764045715No Hit
GGATAACCACATTGTGTAAAAGGAGCAGCAAAACCCAACAGACCCACGAT8480.1616334123710554No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCATCTTTTC8430.16068038517547137No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT8430.16068038517547137No Hit
CGTTAATATGGGCCTAAAGTTCAGACAACTATTGTGGTTTCACATTTCTT8240.15705888183225197No Hit
CTCTATGGAGGGCGGGTGTATTATATCAGAGAGAAACAACACATAGCGCC8240.15705888183225197No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTGAAACATTCTTTGATTTTCTGT8130.15496222200196705No Hit
GAGTAACCCCATCTTTTCGTTTTGTTAGGGTTTAAATGTATACCCAGAGA8040.15324677304991574No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7920.15095950778051404No Hit
GTCCTAGGAATCCTGATGTGATGTTCTCCATGTTCAGCGCAGGGTCCCCA7650.14581316092436014No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT7640.14562255548524333No Hit
CTTTAACCCTCATTGGAAAACACCATCTTTTCCTAATATACATTTACACC7330.1397137868726222No Hit
GATTATGCCTGCTAGGTTTTATCCAAAGGTTACCAAATATTTGCCATTGG7280.13876075967703816No Hit
GAACCACCGTGTGTCTTGGCCAAAATTCGCAGTGCCCAACCTCCAATCAC7070.13475804545558512No Hit
GTAGTCGGTTATGTTAACGTTAATATGGGCCTAAAGTTCAGACAACTATT7050.1343768345773515No Hit
GTGTTGTTTCTCTCTGATATAATACACCCGCCCTCCATAGAGTGTGTAAA7040.1341862291382347No Hit
CCTTTATACCGCTGTTACCAATTTTCTTGTGTCTCTGGGTATACATTTAA7020.13380501826000107No Hit
GTTTTATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC6920.13189896386883296No Hit
CATCCAGAGACCTAGTAGTCGGTTATGTTAACGTTAATATGGGCCTAAAG6890.13132714755148253No Hit
CTACATGTTCGGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATAT6690.12751503876914633No Hit
CTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC6610.12599019525621183No Hit
CTCTGATATAATACACCCGCCCTCCATAGAGTGTGTAAATAATGTCTGGT6350.12103445383917476No Hit
GGATAAGGGTATTAAACCTTATTATCCCGAACATGTAGTTAATCATTACT6330.12065324296094114No Hit
ATAATACACCCGCCCTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG6320.12046263752182433No Hit
CTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAA6290.11989082120447388No Hit
CCTCCGGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6260.11931900488712345No Hit
CTTTATACCGCTGTTACCAATTTTCTTGTGTCTCTGGGTATACATTTAAA6220.11855658313065622No Hit
TCTAACAACAGTAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTG6210.1183659776915394No Hit
GTCTTTAACCCTCATTGGAAAACACCATCTTTTCCTAATATACATTTACA6210.1183659776915394No Hit
AGCCGGAGGAGTGCGAATCCACACTCCGAAAGATACCAAATACTCTAAGA6170.11760355593507216No Hit
GATATAATACACCCGCCCTCCATAGAGTGTGTAAATAATGTCTGGTTTGG6140.11703173961772173No Hit
CGGTAGTCCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC6120.11665052873948811No Hit
CTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAC6100.1162693178612545No Hit
GGATAAGGGTATTAAACCTTATTACCCCGAACATGTAGTTAATCATTACT6080.11588810698302088No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAA6070.11569750154390407No Hit
CCCATGAATATGGTGACCCACAGAATGAGGCGCTATGTGTTGTTTCTCTC6070.11569750154390407No Hit
GGGATACTCTTTACATTTCATGGGCTATGTCATTGGAGGTTATGGGTCAT5880.11207599820068466No Hit
CTCTAGATGTGTCATCTTGCAAATTACCACCCACCCAGGTGGCTAGAGTC5860.11169478732245104No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG5840.11131357644421742No Hit
GAATCAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAAC5730.1092169166139325No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG5720.10902631117481569No Hit
GAAGATGAGGCATAGCAGCAGGATGAAGAGGAAGATGATAAAACGCCGCA5710.10883570573569888No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5710.10883570573569888No Hit
GTTATTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCG5660.10788267854011481No Hit
GTCAATGAGAAACGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC5660.10788267854011481No Hit
GTCTTTGAAGTGTGCCTCAAGGTCGGTCGTTGGCAGAACAGAGAGTCCAA5600.10673904590541396No Hit
CATCTAGAGACCTAGTAGTCGGTTATGTTAACGTTAATATGGGCCTAAAG5600.10673904590541396No Hit
CATTTAAACCCTAACAAAACGAAAAGATGGGGTTACTCTTTACATTTCAT5600.10673904590541396No Hit
GTTCCTGTCTTTAACCCTCATTGGAAAACACCATCTTTTCCTAATATACA5520.10521420239247947No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCTTCCACCAACCGACAGTC5480.10445178063601224No Hit
CTCACAGTCAATGAGAAACGAAGACTGCAATTGATTATGCCTGCTAGGTT5430.10349875344042817No Hit
GTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGGTTAAAGA5400.10292693712307774No Hit
CTTTTTACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAA5400.10292693712307774No Hit
GTAGCTCTTGTTCCCAAGAATATGGTGACCCACAGAATGAGGCGCTATGT5370.10235512080572731No Hit
CTTCGTTTCTCATTGACTGTGAGTGGGCCTACAAATTGTTCACATTTTTT5340.10178330448837689No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCATCTTTTCCT5340.10178330448837689No Hit
GTTCAGCTCACCATACCGCACTCAGGCAAGCAATTCTATGCTGGGGGGAA5310.10121148817102645No Hit
TAATACACCCGCCCTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5300.10102088273190964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)