FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490906
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39130.7970976113553306No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA24400.4970401665491968No Hit
TTCTAAAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA15810.32205758332552464No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT13710.2792795362044872No Hit
CCTTTATACCGCTGTAACCAATTTTCTTTTGTCTTTGGGTATACATTTAA11340.23100145445360212No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT11310.23039033949473015No Hit
TTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10600.2159272854680937No Hit
ATATAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGC10540.21470505555034974No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT9890.20146423144145723No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT9810.19983459155113198No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT9780.19922347659226003No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT9600.19555678683902825No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC9590.1953530818527376No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG9010.18353819264787963No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG8560.17437146826480018No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGGAAAACCCC8410.17131589347044038No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACTACACATAGCGCC8080.16459362892284876No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8080.16459362892284876No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7850.15990841423816374No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT7490.15257503473170014No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7390.1505379848687936No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT7310.1489083449784684No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA7310.1489083449784684No Hit
CTTTTACACAATGCGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT7140.14544536021152726No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT7060.14381572032120202No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAGCATTCTTTGATTTTCTGT6980.1421860804308768No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT6850.13953791560909828No Hit
GTGCTGGTGCGCACACCAATTTATGCCTACAGCCTCCTAATACAAACAAC6790.13831568569135436No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTATACCGCTGTAACCAATTTTCTT6540.133223061034088No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6420.13077860119860013No Hit
CTACTGTACCTGTCTTTAACCCTTATTGGAAAACACCCTCTTTTCCTAAT6190.1260933865139151No Hit
GTTTTAGAATATCTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5880.11977853193890481No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT5850.11916741698003283No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC5700.11611184218567301No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5650.11509331725421976No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATAAGGGTTAAAGA5560.11325997237760387No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG5520.11244515243244126No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5460.1112229225146973No Hit
CTTTAGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA5400.11000069259695339No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCGCATTGTGTAAAA5310.1081673477203375No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA5230.10653770783001226No Hit
GTATAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCCAATACCACATC5090.10368583802194309No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG5090.10368583802194309No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA5080.10348213303565244No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5050.10287101807678048No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCA5030.10246360810419917No Hit
GTCTTTAACCCTTATTGGAAAACACCCTCTTTTCCTAATATACATTTACA5010.10205619813161786No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTGGGCAAGAATTGGTGGGCGTTC4970.10124137818645525No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)