FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490906
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38950.7934309216020989No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA23300.4746326180572248No Hit
TTCTAAAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA14580.29700187001177414No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT12590.2564645777399339No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT11690.23813112897377503No Hit
CCTTTATACCGCTGTAACCAATTTTCTTTTGTCTTTGGGTATACATTTAA10440.2126680056874432No Hit
TTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10350.21083466081082736No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG10210.20798279100275818No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC9680.1971864267293535No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT9550.19453826190757498No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9530.19413085193499366No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT9480.1931123270035404No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT8770.17864927297690392No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8730.1778344530317413No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT8540.1739640582922189No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT8350.17009366355269645No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACTACACATAGCGCC8310.16927884360753384No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGGAAAACCCC7750.15787136437525717No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7720.15726024941638522No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAGCATTCTTTGATTTTCTGT7440.1515565098002469No Hit
GTGCTGGTGCGCACACCAATTTATGCCTACAGCCTCCTAATACAAACAAC7360.14992686990992166No Hit
ATATAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGC7130.1452416552252366No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTATACCGCTGTAACCAATTTTCTT6970.14198237544458614No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT6840.13933421062280762No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC6830.13913050563651697No Hit
CTTTAGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA6750.13750086574619175No Hit
CTTTTACACAATGCGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT6720.13688975078731977No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6480.13200083111634406No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT6190.1260933865139151No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6090.12405633665100854No Hit
CTACTGTACCTGTCTTTAACCCTTATTGGAAAACACCCTCTTTTCCTAAT6050.12324151670584592No Hit
GTTTTAGAATATCTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5900.12018594191148611No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5730.11672295714454499No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5670.11550072722680106No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5500.11203774245985994No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5470.11142662750098797No Hit
GGGTAGTAATTTGGAAGATCCGGTATCCAGGGACCTAGTAGTCAGTTATG5330.1085747576929188No Hit
GTCTTTAACCCTTATTGGAAAACACCCTCTTTTCCTAATATACATTTACA5280.10755623276146555No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT5180.10551918289855898No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5140.10470436295339637No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA5130.10450065796710573No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCA5070.10327842804936178No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATAAGGGTTAAAGA4970.10124137818645525No Hit
GTATAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCCAATACCACATC4960.1010376732001646No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)