FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498594
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTTAT41160.825521366081421No Hit
GTATACATGCATACAAGGGCTTTAAAGCAGGATAACCACATTGTGTAAAA38840.778990521346025No Hit
CATCTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTTAT32820.6582510018171097No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG31260.6269630200122744No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC23510.4715259309177407No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC23350.46831690714288576No Hit
CAATCGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT23190.46510788336803094No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT22970.4606954756776054No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGCTGACAGACT19420.3894952606730125No Hit
GTGCAAGTATTGTTTACACAGAAAGGCCTTATAAGTTGGCGAGAAAGTGA18560.37224675788316747No Hit
GGCCTATTGATTGGAAAGTCTGTCAGCGTATTGTGGGTCTTTTGGGTTTT18150.36402363446010183No Hit
GCGTTCTATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGT18010.36121573865710377No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17740.35580051103703614No Hit
GGAATTAGAGGACAAACGGGCAACATACCTTGGTAGTCCAGAAGAACCAA16500.33093057678191073No Hit
GGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCC15270.3062612065127137No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC14420.2892132677087972No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA14180.2843997320465148No Hit
CATGTATACAGTCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCC13350.26775292121445504No Hit
GTGCAGGTATTGTTTACACAGAAAGGCCTTATAAGTTGGCGAGAAAGTGA13280.26634897331295604No Hit
CATCCAGCGATAACCAGGACAAGTTGGAGGACAGGAGGTTGGTGAGTGAT13220.2651455893973855No Hit
GCATGTTCCCGTGCTGGTAGTTGAAGATCCTGGAATTAGAGGACAAACGG13190.26454389743960016No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT12990.26053261772103153No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAAAGCCCTTGTATGCATGTATA12850.25772472191803353No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT12640.25351287821353646No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC12620.2531117502416796No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA12590.25251005828389433No Hit
GATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAGTTGGAGGAC12470.25010329045275315No Hit
CCCATAACTTCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCATC11860.23786888731111888No Hit
GGGTAAAGGTGCAGGTATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA11500.23064858381769537No Hit
AGCCCGAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGC11440.22944519990212475No Hit
ACAATACGCTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTT11390.22844237997248262No Hit
GCTTAGACTGTATACATGCATACAAGGGCTTTAAAGCAGGATAACCACAT11360.22784068801469734No Hit
TGCTTAGACTGTATACATGCATACAAGGGCTTTAAAGCAGGATAACCACA11210.22483222822577087No Hit
GAATTAGAGGACAAACGGGCAACATACCTTGGTAGTCCAGAAGAACCAAC11070.2220243324227728No Hit
ATAACTTCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCATCTTT10990.2204198205353454No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACTTCCAATGACATAGCCC10900.2186147446619895No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT10710.21480402892934933No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG10540.21139444116856598No Hit
TGGGCATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTA10460.20978992928113857No Hit
CGCTCGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCT10180.20417413767514248No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT10120.2029707537595719No Hit
GTCCCCAACCTCCAATCACTCACCAACCTCCTGTCCTCCAACTTGTCCTG10100.20256962578771506No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGAAGTTATGGGTCATTGCCAC10050.20156680585807293No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC9980.20016285795657388No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA9820.196953834181719No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA9700.19454706635057784No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG9690.1943465023646494No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG9660.19374481040686412No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC9400.18853014677272492No Hit
CTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG9210.1847194310400847No Hit
GGGTTACTCTTTACATTTTATGGGCTATGTCATTGGAAGTTATGGGTCAT8940.17930420342001707No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT8930.17910363943408866No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG8760.17569405167330532No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC8650.1734878478280926No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG8630.17308671985623575No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA8250.16546528839095537No Hit
GTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTG8170.16386077650352793No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTGCAGGTATTGTTTACAC8170.16386077650352793No Hit
CTGTAACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA8120.16285795657388577No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8030.1610528807005299No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA7910.15864611286938873No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA7750.15543708909453383No Hit
ATGTGATCTTGTGGCAATGACCCATAACTTCCAATGACATAGCCCATAAA7720.15483539713674854No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG7560.15162637336189366No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG7520.15082411741817991No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG7520.15082411741817991No Hit
CAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGCCTATTGA7480.1500218614744662No Hit
CTTTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAA7390.14821678560111032No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG7390.14821678560111032No Hit
GTCCCGTGCTGGTAGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC7310.14661227371368288No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG7240.14520832581218387No Hit
CATGCATACAAGGGCTTTAAAGCAGGATAACCACATTGTGTAAAAGGGGC7200.14440606986847013No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT7160.1436038139247564No Hit
GTCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCCTTTCTGTGTA7070.14179873805140053No Hit
ACCCATAACTTCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCAT6980.13999366217804465No Hit
ATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT6900.1383891502906172No Hit
TGTTTACACAGAAAGGCCTTATAAGTTGGCGAGAAAGTGAAAGCCTGCTT6860.1375868943469035No Hit
ATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACCTG6820.13678463840318977No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6730.1349795625298339No Hit
GGATGTCATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGA6610.13257279469869274No Hit
GACATAGCCCATAAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAG6580.13197110274090743No Hit
CTGTATACATGCATACAAGGGCTTTAAAGCAGGATAACCACATTGTGTAA6580.13197110274090743No Hit
GGCCGATTGATTGGAAAGTCTGTCAGCGTATTGTGGGTCTTTTGGGTTTT6490.13016602686755155No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA6470.1297648988956947No Hit
GCCCAGGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTT6440.12916320693790942No Hit
GATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAA6420.12876207896605252No Hit
GCCCATGACCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC6360.12755869505048195No Hit
CGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCT6300.12635531113491136No Hit
ATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATAC6290.12615474714898295No Hit
GGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTAC6290.12615474714898295No Hit
ATGTTTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCT6280.1259541831630545No Hit
CTCTACTTCCAGGATCTTCAACTACCAGCACGGGACCATGCAGAACCTGC6180.12394854330377021No Hit
ATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCT6060.12154177547262902No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT5940.11913500764148786No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC5890.11813218771184571No Hit
CTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC5870.11773105973998885No Hit
AGATCACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA5820.1167282398103467No Hit
ACCCAAAAGACCCACAATACGCTGACAGACTTTCCAATCAATAGGCCTGT5810.11652767582441828No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5720.11472259995106238No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTACCAAGGTATGTTGC5690.1141209079932771No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC5690.1141209079932771No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC5680.11392034400734867No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC5660.11351921603549181No Hit
TCGTAGGGCTTTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGG5630.1129175240777065No Hit
GCTTTAAAGCCCTTGTATGCATGTATACAGTCTAAGCAGGCTTTCACTTT5610.11251639610584964No Hit
GGCTATGTCATTGGAAGTTATGGGTCATTGCCACAAGATCACATCATACA5590.1121152681339928No Hit
GTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACCAACCTCC5580.11191470414806436No Hit
GTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTCA5580.11191470414806436No Hit
CCCATAGGAATTTTCCGAAAGCCCAGGATGATGGGATGGGAATACAGGTG5530.1109118842184222No Hit
CCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAG5460.1095079363169232No Hit
CTCTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACA5380.10790342442949576No Hit
GCATACAAGGGCTTTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGC5370.1077028604435673No Hit
GTTCTATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTG5360.10750229645763887No Hit
ACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGG5350.10730173247171045No Hit
ATGTGGTTATCCTGCTTTAAAGCCCTTGTATGCATGTATACAGTCTAAGC5350.10730173247171045No Hit
CCTTATAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGACTGTATACATGCA5350.10730173247171045No Hit
CTCCCATAGGAATTTTCCGAAAGCCCAGGATGATGGGATGGGAATACAGG5180.10389214471092711No Hit
AGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAATTT5160.10349101673907027No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTTACAGCGGTAAAAA5150.10329045275314183No Hit
GGATGTCATGGGTCATTGCCACAAGATCACATCATACAGAGAATCAAAGA5120.10268876079535655No Hit
GCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAA5080.10188650485164281No Hit
GCCTTATAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGACTGTATACATGC5070.10168594086571439No Hit
GCCAGAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCC5050.10128481289385753No Hit
GCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCTGGCC5050.10128481289385753No Hit
GTTCAGGAACAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCA5010.1004825569501438No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)