FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498594
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTTAT43610.8746595426338865No Hit
GTATACATGCATACAAGGGCTTTAAAGCAGGATAACCACATTGTGTAAAA39070.7836034930223789No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG36140.7248382451453487No Hit
CATCTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTTAT33360.669081457057245No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC25040.5022122207647907No Hit
CAATCGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT23730.47593833860816614No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC23250.4663112672836015No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT22920.4596926557479633No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGCTGACAGACT20740.41596970681556533No Hit
GGCCTATTGATTGGAAAGTCTGTCAGCGTATTGTGGGTCTTTTGGGTTTT19750.39611387220865074No Hit
GCGTTCTATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGT19650.3941082323493664No Hit
GTGCAAGTATTGTTTACACAGAAAGGCCTTATAAGTTGGCGAGAAAGTGA18700.3750546536861655No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17690.354797691107394No Hit
GGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCC15910.31909730161213334No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC14800.2968346991740775No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA14590.2926228554695805No Hit
GTGCAGGTATTGTTTACACAGAAAGGCCTTATAAGTTGGCGAGAAAGTGA14410.2890127037228687No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT13540.27156363694709523No Hit
GGAATTAGAGGACAAACGGGCAACATACCTTGGTAGTCCAGAAGAACCAA13110.2629393855521727No Hit
GTCCCCAACCTCCAATCACTCACCAACCTCCTGTCCTCCAACTTGTCCTG13020.26113430967881684No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC12930.25932923380546097No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAAAGCCCTTGTATGCATGTATA12900.25872754184767566No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACTTCCAATGACATAGCCC12700.2547162621291071No Hit
CATGTATACAGTCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCC12540.25150723835425215No Hit
CGCTCGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCT12230.2452897547904708No Hit
GCATGTTCCCGTGCTGGTAGTTGAAGATCCTGGAATTAGAGGACAAACGG12040.24147903905783064No Hit
GGGTAAAGGTGCAGGTATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA12030.2412784750719022No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT11940.2394733991985463No Hit
CCCATAACTTCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCATC11700.23465986353626397No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT11480.2302474558458385No Hit
AGCCCGAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGC11450.22964576388805322No Hit
CATCCAGCGATAACCAGGACAAGTTGGAGGACAGGAGGTTGGTGAGTGAT11240.22543392018355615No Hit
GCTTAGACTGTATACATGCATACAAGGGCTTTAAAGCAGGATAACCACAT11150.2236288443102003No Hit
TGCTTAGACTGTATACATGCATACAAGGGCTTTAAAGCAGGATAACCACA11030.22122207647905912No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA10930.21921643661977483No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT10880.21821361669013264No Hit
ATAACTTCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCATCTTT10660.21380120899970717No Hit
GATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAGTTGGAGGAC10550.21159500515449442No Hit
ACAATACGCTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTT10540.21139444116856598No Hit
TGGGCATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTA10430.2091882373233533No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGAAGTTATGGGTCATTGCCAC10380.2081854173937111No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC10370.20798485340778267No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG10230.20517695760478466No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA10150.2035724457173572No Hit
GAATTAGAGGACAAACGGGCAACATACCTTGGTAGTCCAGAAGAACCAAC10090.20236906180178663No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG9960.19976172998471703No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA9950.1995611659987886No Hit
GTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTG9590.19234086250536508No Hit
CTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG9480.19013465866015236No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC9460.1897335306882955No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC9390.18832958278679646No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG9160.18371661111044257No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG9100.18251322719487198No Hit
GGGTTACTCTTTACATTTTATGGGCTATGTCATTGGAAGTTATGGGTCAT8970.17990589537780238No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT8870.17790025551851807No Hit
CTGTAACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA8760.17569405167330532No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTGCAGGTATTGTTTACAC8490.1702788240532377No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG8320.16686923629245437No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG8150.16345964853167105No Hit
ATGTGATCTTGTGGCAATGACCCATAACTTCCAATGACATAGCCCATAAA8020.16085231671460146No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG7960.15964893279903086No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA7880.15804442091160342No Hit
ATGTTTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCT7860.15764329293974658No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG7850.15744272895381814No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA7830.1570416009819613No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG7720.15483539713674854No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA7710.1546348331508201No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG7530.15102468140410835No Hit
ATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT7350.1474145296573966No Hit
CTTTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAA7340.14721396567146816No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT7310.14661227371368288No Hit
CGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCT7260.14560945378404072No Hit
GTCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCCTTTCTGTGTA7230.14500776182625544No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7150.143403249938828No Hit
GTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACCAACCTCC7080.14199930203732897No Hit
GGCCGATTGATTGGAAAGTCTGTCAGCGTATTGTGGGTCTTTTGGGTTTT7010.14059535413582994No Hit
GGATGTCATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGA6990.1401942261639731No Hit
ATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATAC6880.13798802231876037No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC6820.13678463840318977No Hit
TGTTTACACAGAAAGGCCTTATAAGTTGGCGAGAAAGTGAAAGCCTGCTT6780.13598238245947605No Hit
ACCCATAACTTCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCAT6760.13558125448761918No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6760.13558125448761918No Hit
CAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGCCTATTGA6720.13477899854390546No Hit
GTCCCGTGCTGGTAGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC6710.13457843455797702No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT6680.13397674260019174No Hit
GCCCAGGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTT6630.13297392267054958No Hit
GCCCATGACCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC6630.13297392267054958No Hit
ATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACCTG6530.1309682828112653No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA6520.13076771882533686No Hit
CATGCATACAAGGGCTTTAAAGCAGGATAACCACATTGTGTAAAAGGGGC6460.12956433490976627No Hit
ATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCT6430.12896264295198098No Hit
AGATCACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA6330.12695700309269664No Hit
CTCTACTTCCAGGATCTTCAACTACCAGCACGGGACCATGCAGAACCTGC6320.12675643910676823No Hit
GTTCAGGAACAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCA6310.1265558751208398No Hit
CTGTATACATGCATACAAGGGCTTTAAAGCAGGATAACCACATTGTGTAA6200.12434967127562706No Hit
GACATAGCCCATAAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAG6190.12414910728969863No Hit
ATGTGGTTATCCTGCTTTAAAGCCCTTGTATGCATGTATACAGTCTAAGC6180.12394854330377021No Hit
GCTTTAAAGCCCTTGTATGCATGTATACAGTCTAAGCAGGCTTTCACTTT6100.12234403141634276No Hit
CTCTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACA6040.12114064750077216No Hit
GTTCTATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTG6040.12114064750077216No Hit
TCGTAGGGCTTTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGG5970.11973669959927315No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC5890.11813218771184571No Hit
CTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC5850.11732993176813199No Hit
GATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAA5750.11532429190884769No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTACCAAGGTATGTTGC5730.11492316393699083No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC5620.11271696009177808No Hit
ACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGG5490.11010962827470848No Hit
GGCTATGTCATTGGAAGTTATGGGTCATTGCCACAAGATCACATCATACA5480.10990906428878006No Hit
CATTGGAAGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCA5480.10990906428878006No Hit
GTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTCA5470.10970850030285163No Hit
CTCCCATAGGAATTTTCCGAAAGCCCAGGATGATGGGATGGGAATACAGG5460.1095079363169232No Hit
GCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAA5360.10750229645763887No Hit
GCCCATAAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTA5360.10750229645763887No Hit
GGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTAC5350.10730173247171045No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC5340.10710116848578202No Hit
GCCAGAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCC5340.10710116848578202No Hit
CCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGG5340.10710116848578202No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5280.10589778457021143No Hit
ACCCAAAAGACCCACAATACGCTGACAGACTTTCCAATCAATAGGCCTGT5250.10529609261242615No Hit
ATACCTGCACCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAG5200.104293272682784No Hit
CCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAG5160.10349101673907027No Hit
CCCATAGGAATTTTCCGAAAGCCCAGGATGATGGGATGGGAATACAGGTG5150.10329045275314183No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC5140.10308988876721341No Hit
GCTCAAGGAACCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGA5030.10088368492200067No Hit
AGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAATTT5000.10028199296421537No Hit
GCCTTATAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGACTGTATACATGC4990.10008142897828694No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)