FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556508
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40250.7232600429823112No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA33170.5960381521918823No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT13890.24959209930495158No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT13290.23881058313627118No Hit
CTCTAAGACCGTATTTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA12680.22784937503144612No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12410.2229976927555399No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT11080.1990986652482983No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAAACT10950.19676267007841755No Hit
ATCCTAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT10540.18939530069648597No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT10460.18795776520732854No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC9910.17807470871937153No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT9410.16909011191213782No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC9030.16226181833864023No Hit
CAACAGAGTTATCCGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCA8500.15273814572297253No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA8080.14519108440489625No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7690.138183098895254No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTTTTGTGGTTTCACATTTCTT7530.1353080279169392No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT7530.1353080279169392No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7380.1326126488747691No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT7370.13243295693862442No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAGTT7020.12614373917356084No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6990.12560466336512682No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTAGGATAAAAC6780.12183113270608867No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT6680.12003421334464194No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC6610.11877636979162924No Hit
CCTTAGGATAAAACCTAGCAGGCATAATTAATTGTAGTCTTCTTTTTTCA6520.11715914236632716No Hit
GTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGTGTGATGTGAT6410.11518253106873576No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC6310.11338561170728902No Hit
ACTGTGCAGTTACTCTCGTTTTTGCCTACTGACTTCTTTCCTTCAGTACG6260.11248715202656565No Hit
GACCCATAGCATCCAATGACATAGCCCATGAAATTTAAAGAGTAACCCCA6200.11140900040969762No Hit
CTTTTACACAATGTGGTTATCCTGCTCTCATGCCCTTGTATGCCTGTATT6030.10835423749523818No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA5920.10637762619764676No Hit
GGATAAAACCTAGCAGGCATAATTAATTGTAGTCTTCTTTTTTCATTGAC5910.10619793426150208No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT5850.10511978264463404No Hit
ACTAAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGG5790.10404163102776601No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG5780.10386193909162132No Hit
GACCAGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTTTACAC5650.10152594392174057No Hit
AAACTAAAAGATGGGGTTACTCTTTAAATTTCATGGGCTATGTCATTGGA5570.10008840843258318No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)