FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581434
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA59871.0296955458401127No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46970.8078302954419591No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT28890.4968749677521438No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT27150.4669489572333231No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG25060.43100334689749825No Hit
AGAGTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT20810.3579082062624477No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA19920.3426012238706371No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC19720.3391614525466347No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT16330.28085732860479434No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT15650.26916210610318625No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT15390.2646904033819832No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT13800.23734422135616423No Hit
TTGTTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGC13680.23528035856176283No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT12520.21532968488254903No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT12120.20845014223454422No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT11860.20397843951334116No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT11620.19985071392453832No Hit
CTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA11050.1900473656511315No Hit
GTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTA9720.1671728863465157No Hit
ATAGTATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG9700.16682890921411545No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9590.16493703498591414No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9340.16063732083091117No Hit
ATATTTGCCATTGGATAAGGGTATTAAACCTTATTATCCAGAACATCTAG8720.14997402972650378No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG8510.14636226983630127No Hit
CACTAATATGGGCCTAAAGTTTAGACAACTATTGTGGTTTCACATTTCTT8490.14601829270390104No Hit
GCAATTGATTATGCCTGCTAGGTTTTATCCAAAGGTTACCAAATATTTGC8430.1449863613067003No Hit
CCCTCACAAAACAAAAAGATGGGGTTACTCTTTACATTTCATGGGCTATG8330.14326647564469913No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAATCAACACGTAGCGCC8000.1375908529600952No Hit
GGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAA7410.1274435275542882No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT7410.1274435275542882No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAAGGTTA7390.12709955042188795No Hit
TATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAA7260.12486369906128642No Hit
CAATTGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT7180.12348779053168546No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTAATTGGAAAACACCCTCTTTTC7020.12073597347248355No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA7010.12056398490628342No Hit
ATTTAGTGGTCTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACA6990.1202200077738832No Hit
GTGTAAAAGGTGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT6780.1166082478836807No Hit
ATATAGTATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA6750.11609228218508033No Hit
GTTTTATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC6720.11557631648647998No Hit
CATTAGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCAT6690.11506035078787961No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC6660.11454438508927928No Hit
ATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACAC6520.1121365451624776No Hit
GATTATGCCTGCTAGGTTTTATCCAAAGGTTACCAAATATTTGCCATTGG6500.11179256803007737No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG6420.11041665950047641No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTAATTGGAAAACACCCT6410.11024467093427628No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGATAATAAGGTTTAATAC6260.10766484244127451No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCCACCGTG6190.10646092247787367No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG6140.10560097964687307No Hit
CTTTAAATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA6120.10525700251447284No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACACACTCTATGGA5960.10250518545527093No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5960.10250518545527093No Hit
CCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGAT5910.10164524262427034No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)