FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581434
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA60291.0369190656205176No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46810.805078478382757No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT28350.4875875851773374No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT25260.4344431182215006No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG24320.4182761929986895No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA21050.3620359318512505No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC21050.3620359318512505No Hit
AGAGTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT19500.33537770409023204No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT17410.29943209375440716No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT15830.27225790029478836No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT14800.25454307797617615No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT14170.24370779830556866No Hit
TTGTTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGC13790.23717223278996413No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT11790.20277451954994033No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT11790.20277451954994033No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT11440.19675491973293616No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT11210.19279918271033342No Hit
CTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA10970.18867145712153055No Hit
ATAGTATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG9980.1716445890677188No Hit
GTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTA9670.16631294351551507No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9520.1637331150225133No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG9280.15960538943371044No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8970.15427374388150675No Hit
CCCTCACAAAACAAAAAGATGGGGTTACTCTTTACATTTCATGGGCTATG8430.1449863613067003No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC8260.1420625556812983No Hit
ATATTTGCCATTGGATAAGGGTATTAAACCTTATTATCCAGAACATCTAG8230.14154658998269795No Hit
ATTTAGTGGTCTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACA7880.13552699016569378No Hit
GCAATTGATTATGCCTGCTAGGTTTTATCCAAAGGTTACCAAATATTTGC7810.13432307020229295No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAATCAACACGTAGCGCC7590.13053932174589034No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT7560.13002335604728996No Hit
CACTAATATGGGCCTAAAGTTTAGACAACTATTGTGGTTTCACATTTCTT7540.12967937891488973No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA7290.12537966475988677No Hit
GGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAA7240.12451972192888618No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCCACCGTG7110.12228387056828462No Hit
CAATTGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT6980.12004801920768307No Hit
TATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAA6960.11970404207528283No Hit
GTGTAAAAGGTGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT6930.11918807637668247No Hit
ATATAGTATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA6920.11901608781048237No Hit
CATTAGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCAT6800.11695222501608092No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTAATTGGAAAACACCCTCTTTTC6720.11557631648647998No Hit
GTTTTATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC6680.1148883622216795No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG6590.11334046512587843No Hit
GATTATGCCTGCTAGGTTTTATCCAAAGGTTACCAAATATTTGCCATTGG6510.11196455659627748No Hit
ATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACAC6460.11110461376527689No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTAATTGGAAAACACCCT6270.10783683100747461No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAAGGTTA6240.10732086530887426No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACACACTCTATGGA6220.10697688817647402No Hit
ATCCAAAAGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT6190.10646092247787367No Hit
CTTTAAATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA6010.10336512828627152No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG5850.10061331122706962No Hit
CCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGAT5840.10044132266086951No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGATAATAAGGTTTAATAC5830.10026933409466939No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA5830.10026933409466939No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)