FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589023
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA58420.9918118647319375No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28390.48198457445634546No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT24600.4176407372886967No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC21980.37316030104087616No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC21230.36042735173329393No Hit
ATCTCGGGACTCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAAC18540.31475850688343243No Hit
CTCTATGGAAGGCGGGTATATTATACAAGAGAGAAACAACACATAGCGCC17250.292857834074391No Hit
CTCTCAATTTTATGGGTTATGTCATTGGATGTCATGGATCATTGCCACAA16170.2745223870714726No Hit
TCCCTAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCG15550.26399648231053796No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT15010.25482875880907874No Hit
ATTCTTTGACACACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCT14780.2509239876880869No Hit
GTATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA14660.24888671579887373No Hit
GATAAAACGCCGCAGACACATCCAACGATAACCAGGACAAGTTGGAGGAC14430.24498194467788187No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG12670.21510195696942225No Hit
TTCTATAACTGTTTGTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA12210.20729241472743848No Hit
AATTTAGAGAATAACCCCATCTCTTTGTTTTGTTAGGGTTTAAATGTATA11750.1994828724854547No Hit
GTTTAATACCCTTCTCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC11410.19371060213268412No Hit
CTTGTATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTT10620.1802985621953642No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGAGAAGGGTATTAAACCTTAT10450.17741242701897889No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTCTAAATTTTAT10290.174696064500028No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT10210.17333788324055258No Hit
TTCTACAACTGTTTGTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10170.17265879261081488No Hit
GTGAAAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAA9600.16298175113705238No Hit
ATCTAGGGACTCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAAC9510.16145379722014253No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTAT9490.16111425190527365No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9460.16060493393297037No Hit
GTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCTTTGACACACT9410.15975607064579822No Hit
CACTAATATGGGCCTAAAGTTCAGACAACTCTTGTGGTTTCACATTTCTT9400.15958629798836377No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCCTTTCCAAAT9270.15737925344171622No Hit
GGATAATAAGGTTTAATACCCTTCTCCAATGGCAAATATTTGGTAACCTT9230.1567001628119785No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTA8860.1504185744869046No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCACC8780.14906039322742914No Hit
CTATTTACACACTCTATGGAAGGCGGGTATATTATACAAGAGAGAAACAA8740.14838130259769142No Hit
GTTATAGAATATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC8700.14770221196795372No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTAGT8550.14515562210643726No Hit
GCGTTCTATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGT8440.14328812287465856No Hit
TTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTATG7930.13462971734550264No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7880.13378085405833048No Hit
GTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGG7590.12885744699273205No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCCTTTC7580.12868767433529762No Hit
GGCCTCTCACTCTGGGATCTTGCAGAGTTTGATGGAAGGTTGTGGAGTTC7540.1280085837055599No Hit
GATTATGCCTGCTAGGTTTTATCCAAAGGTTACCAAATATTTGCCATTGG7360.12495267587174015No Hit
ATCCAACATCTAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGGC7310.12410381258456801No Hit
CAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTAT7260.12325494929739586No Hit
CATCAAGACCTCATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT7240.122915403982527No Hit
AGACCTCATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGTTAATG7210.12240608601022372No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCT7050.11968972349127283No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6940.11782222425949411No Hit
GTCCCGTGCTGGTGGTTGAGGATCCTGGAATTAGAGGACAAACGGGCAAC6930.11765245160205968No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGCTC6920.11748267894462525No Hit
GCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACTGCACTCAGGCA6900.1171431336297564No Hit
ACACCAAATATTCTATAACTGTTTGTCTTCCAAAAGTGAGACAAGAAATG6900.1171431336297564No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC6890.11697336097232197No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6810.11561517971284653No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTG6800.1154454070554121No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC6730.11425699845337108No Hit
GTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTATCC6730.11425699845337108No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTTCTGTCGA6680.11340813516619894No Hit
GTTCTGGATAATAAGGTTTAATACCCTTCTCCAATGGCAAATATTTGGTA6670.11323836250876451No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6520.11069177264724807No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6500.11035222733237922No Hit
CTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCCTTTCCA6350.10780563747086276No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6300.10695677418369061No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGCTCACGGTGGTTTCCATGC6230.10576836558164962No Hit
CTCTAAATTTTATGGGTTATGTCATTGGATGTCATGGATCATTGCCACAA6230.10576836558164962No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG6170.10474972963704303No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6130.10407063900730533No Hit
CTTTAATCCTCATTGGAAAACACCCTCCTTTCCAAATATACATTTACATC6090.1033915483775676No Hit
GTGTTGTTTCTCTCTTGTATAATATACCCGCCTTCCATAGAGTGTGTAAA6000.10186359446065774No Hit
GCCAACCCCGCTGTCTCCACCTTTGCGAGAAACTCATCCTCAGGCCATGC5990.10169382180322331No Hit
GGGTTGGCTGCCTTCCTGACTGGCGATTGGTGGAGGCAGGAGGCGGATTT5930.10067518585861672No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)