FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678015
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN51600.7610451096214685No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCAGG30030.44291055507621513No Hit
ATCCTAACCTTACCAAGTATTTGCCCTTGGATAAAGGCATTAAACCGTAT22670.33435838440152504No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT16930.24969949042425313No Hit
CTCTTACACCTAATGAAAAAAGGAGATTAAACTTAATTATGCCTGCTAGG15850.23377063929264102No Hit
CATTTACAGGAGGACATTATTGATAGATGTCAACAATATGTGGGCCCTCT12830.18922885186905894No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11450.16887531986755455No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA11400.16813787305590583No Hit
CTTACACCTAATGAAAAAAGGAGATTAAACTTAATTATGCCTGCTAGGTT11290.16651549007027866No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAAGTTTAATCTCCTTTTTTCA10120.14925923467769886No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9930.14645693679343377No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9470.13967242612626565No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCA9290.1370176176043303No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA8970.13229795800977856No Hit
GTTCTATCCTAACCTTACCAAGTATTTGCCCTTGGATAAAGGCATTAAAC8310.12256366009601558No Hit
ATTTAAAGGAGGACATTATTGATAGATGTCAACAATATGTGGGCCCTCTT8040.11858144731311256No Hit
GTTTTGAAGTAATGATTAACTGCATTTTCAGGATAATACGGTTTAATGCC7780.11474672389253926No Hit
TCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGG7720.1138617877185608No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA7550.11135446855895519No Hit
GTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTTT7320.10796221322537111No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC6840.1008827238335435No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)