FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678015
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN51430.7585377904618629No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCAGG31030.45765949130918937No Hit
ATCCTAACCTTACCAAGTATTTGCCCTTGGATAAAGGCATTAAACCGTAT20280.2991084268047167No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT15670.23111583077070566No Hit
CTCTTACACCTAATGAAAAAAGGAGATTAAACTTAATTATGCCTGCTAGG14810.21843174561034787No Hit
CATTTACAGGAGGACATTATTGATAGATGTCAACAATATGTGGGCCCTCT12100.17846212841898776No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10850.16002595812777004No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA10710.15796110705515365No Hit
CTTACACCTAATGAAAAAAGGAGATTAAACTTAATTATGCCTGCTAGGTT10570.15589625598253726No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT10150.14970170276468808No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAAGTTTAATCTCCTTTTTTCA10090.14881676659070964No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCA9880.14571948998178508No Hit
GTTTTGAAGTAATGATTAACTGCATTTTCAGGATAATACGGTTTAATGCC9080.1339203409954057No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8630.1272833196905673No Hit
ATTTAAAGGAGGACATTATTGATAGATGTCAACAATATGTGGGCCCTCTT8040.11858144731311256No Hit
TCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGG7890.1163691068781664No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA7760.11445174516787977No Hit
GTTCTATCCTAACCTTACCAAGTATTTGCCCTTGGATAAAGGCATTAAAC7720.1138617877185608No Hit
GTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTTT6840.1008827238335435No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)