FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences573569
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN106641.8592357676234246No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA31420.5477980853219054No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT12520.2182823688170037No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT11700.20398591974112967No Hit
GTACCATTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTT11600.20224245034163282No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT10740.18724861350596006No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG10660.1858538379863626No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC10130.17661345016902935No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA9420.16423481743260182No Hit
CTACTGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTATTAT9420.16423481743260182No Hit
ATCCTACTGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT9240.16109657251350754No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8710.1518561846961743No Hit
CTACCAAAACTAAAAGATGGGGTTACTCTTTAAATTTCATGGGTTATGTC8270.14418491933838823No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT8250.14383622545848887No Hit
CAATTAGGGTTAAAGACAGGTACAGTAGAAGAATAAAGCCCCGTAAAGTT7960.13878016419994804No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7850.13686234786050153No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT7660.13354975600145755No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA7430.12953977638261482No Hit
ATCTTAATGTCAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG7070.12326328654442623No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC6840.1192533069255835No Hit
GTCAACACTAATATGGGCCTAAAGTTCAGGCAACTTTTGTGGTTTCACAT6700.11681244976628792No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT6310.11001291910825027No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6150.10722336806905533No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5970.10408512314996103No Hit
CCCAAAAGACCCACAATACGTTGACAGACTTTCCAATCAATTGGCCGGTT5840.10181861293061514No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)