FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362204
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA31720.8757495775861117No Hit
AATATACACTTACACCAAGACATTATCAAGAAATGTGAACAATTTGTAGG12340.34069198573179754No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12160.3357224105752559No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCCTAT11740.3241267352099922No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT11200.30921800974036734No Hit
GGATAATAGGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT11090.30618104714470296No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT11030.30452452209252245No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAGAAC10070.2780201212576338No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT10050.2774679462402403No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8990.2482026703183841No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8210.22666784464003709No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA8100.2236308820443728No Hit
CACTTACACCAAGACATTATCAAGAAATGTGAACAATTTGTAGGCCCACT7930.21893739439652793No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC7440.20540910647038688No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT7270.200715618822542No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT7050.19464169363121336No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAGAACCTAGCAGG6950.19188081854424577No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6920.19105255601815552No Hit
AGATAAGGGTATTAAACCCTATTATCCAGAACATCTAGTTAATCATTACT6810.1880155934224912No Hit
GTATTAAACCCTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC6660.18387428079203985No Hit
GTTGTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCT6450.17807644310940796No Hit
GTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTT6380.17614383054853067No Hit
GTTCTGGATAATAGGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA6000.1656525052180539No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG5880.16233945511369285No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5860.16178728009629934No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC5760.15902640500933177No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG5560.15350465483539663No Hit
CACCAAGACATTATCAAGAAATGTGAACAATTTGTAGGCCCACTCACAGT5560.15350465483539663No Hit
GGGTAAAGGTTCATGTATTGTTTACACAAAAAGGCCTTGTAAGTTGGCGA5460.15074377974842906No Hit
CTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT5450.1504676922397323No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACACTTACACC5360.1479829046614615No Hit
CTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCT5320.14687855462667448No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG5260.14522202957449393No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5240.1446698545571004No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTACT5230.14439376704840365No Hit
CAATAGGCCTGTTGACAGGAAGTTTTCTAAAACAGTCTTTGATTTTCTGT5140.14190897947013284No Hit
CATTTGGATAGAACCTAGCAGGCATAATTAATTGCAGTCTCCGTTTCTCA5110.1410807169440426No Hit
GTTCTATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAAC5030.13887201687446854No Hit
GCAATTAATTATGCCTGCTAGGTTCTATCCAAATGTTACCAAATATTTGC4910.13555896677010745No Hit
GTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACACTTACA4680.12920895407008204No Hit
CTCATAAGGTGGGAAACTTTACGGGGTTGTATTCTTCTACTGTACCTGTC4400.12147850382657285No Hit
TTCTAAAACAGTCTTTGATTTTCTGTATGATGTGATCTTGTGGCAATGAC4260.11761327870481827No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT4210.1162328411613345No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4200.11595675365263775No Hit
GTCAATGAGAAACGGAGACTGCAATTAATTATGCCTGCTAGGTTCTATCC4170.11512849112654748No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCATA4100.11319587856567018No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTGATTCCAGGATCTTCAACCACC4070.1123676160395799No Hit
TTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAGAACCT4020.11098717849609611No Hit
TAATTATGCCTGCTAGGTTCTATCCAAATGTTACCAAATATTTGCCATTA3950.10905456593521884No Hit
CTATTATGGTTTCACATTTCTTGTCTCACTTTTGGAAGAGAAACGGTCAT3940.10877847842652208No Hit
AATTATGCCTGCTAGGTTCTATCCAAATGTTACCAAATATTTGCCATTAG3900.10767412839173506No Hit
GTCTTGGTGTAAGTGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGAT3900.10767412839173506No Hit
CACTAATATGGGCCTGAAGTTCAGGCAACTATTATGGTTTCACATTTCTT3840.10601760333955451No Hit
GCTAGGTTCTATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTAT3780.10436107828737397No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGTTGTATTCTTCTACTG3760.10380890326998045No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3720.10270455323519344No Hit
GGATAGAACCTAGCAGGCATAATTAATTGCAGTCTCCGTTTCTCATTGAC3690.10187629070910315No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCAGCTACCAATCGCCAGTC3690.10187629070910315No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)