FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501162
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGT46070.9192636313208105No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG43630.8705767795642926No Hit
CCAAAACTGTGCACCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTT38720.772604467218185No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT26510.5289706721579049No Hit
TACCTCGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG25540.5096156532219123No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC23940.47768984879140874No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23340.46571767212996995No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT22590.45075245130317143No Hit
GACAAGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCA22040.4397779560301859No Hit
CTGTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGC21690.4327941863110132No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC19480.3886966689413802No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA18250.3641537067854307No Hit
GGACTCGTGCAGGTTCTGCAGGGTCCCGTGCTGGTGGTTGAAGATCCTGG18140.36195880773083355No Hit
CATCAAACAGAAAATCAAAGAATGTTTTAGGAAACTTCCTGTTAACAGGC18120.36155973517545226No Hit
CCTCTTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGT17670.35258060267937313No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG17150.34220471623945947No Hit
CCTCGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT16870.33661770046412137No Hit
ATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGT16400.327239495412661No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT15950.3182603629165819No Hit
GGTCCTGCAGGGTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGAC15940.31806082663889124No Hit
GGCCAAAACTGTGCACCATCTTGAGTCCCTTTTTACCGCTGTTACCAATT15640.3120747383081718No Hit
GGAGTCGTGCAGGTCCTGCAGGGTCCCGTGCTGGTGGTTGAAGATCCTGG15510.3094807666981934No Hit
GAATACATGCATACAAGGGCTTTAAAGCAGGATAACCACATTGTGTAAAA15170.3026965332567114No Hit
CTGTTTGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGC14960.2985062714252078No Hit
ACTGTGCACCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTT14910.29750859003675456No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGGAAACTTCCTGTTAACAGGC14370.28673363104145966No Hit
GCACGTCTCCTGCTCAAGGAACCTCTATGTATCCCTCCTGTTGCTGTACC13830.2759586720461647No Hit
ATGTGGTATTGGGGGCCAAAACTGTGCACCATCTTGAGTCCCTTTTTACC13460.26857582977161076No Hit
CATGCATACAAGGGCTTTAAAGCAGGATAACCACATTGTGTAAAAGGGGC13390.2671790758277762No Hit
GCCAAAACTGTGCACCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTT12680.25301200011174035No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG12140.24223704111644537No Hit
CTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCA12100.24143889600568277No Hit
CAGTCTTGGCCCCCAATACCACATCATCCATATAACTAAAGGCCAAACAG11900.23744817045186986No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCTGTTGCTGTACC11860.23665002534110727No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT11860.23665002534110727No Hit
ATCGAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC11510.22966625562193463No Hit
GACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAG11300.22547599379043104No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT11220.22387970356890588No Hit
GTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACCTGTA11200.22348063101352458No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT11100.22148526823661813No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT10930.21809315151587713No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT10820.21589825246127997No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA10800.21549917990589867No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAAAGCCCTTGTATGCATGTATT10480.20911401901979798No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA10400.2075177287982728No Hit
ACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA10220.20392607579984118No Hit
GATCCTGTGGCAATGACCCATAACATCCAATGACATAGCCCATGAAATGT10210.20372653952215053No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC10180.20312793068907858No Hit
ACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCAT10180.20312793068907858No Hit
GGAGACGTGCAGGTCCTGCAGGGTCCCGTGCTGGTGGTTGAAGATCCTGG9910.1977404511914311No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT9890.19734137863604984No Hit
GTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCC9800.195545552136834No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTCCTAAAAC9690.1933506530822369No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGGAAAATTCCTATGGGAG9470.18896085497304263No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCG9450.18856178241766136No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG9390.18736456475151747No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC9370.18696549219613617No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA9230.18417198430846712No Hit
CAGTCTTGGCCCCCAATACCACATCATCCATATAACTAAAAGCCAAACAG9020.17998172247696353No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA8980.17918357736620094No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC8720.1739956341462441No Hit
GATTGAATACATGCATACAAGGGCTTTAAAGCAGGATAACCACATTGTGT8690.1733970253131722No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC8670.1729979527577909No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC8620.17200027136933765No Hit
GTTTTGGCCCCCAATACCACATCATCCATATAACTAAAAGCCAAACAGTG8430.16820908209321536No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA8430.16820908209321536No Hit
GCTTAGATTGAATACATGCATACAAGGGCTTTAAAGCAGGATAACCACAT8300.16561511048323696No Hit
TTCCCCCACTGTTTGGCTTTTAGTTATATGGATGATGTGGTATTGGGGGC8240.16441789281709307No Hit
ACCTCGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGA8100.16162438492942402No Hit
ATCAAGGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATCTTCAACCACC8050.1606267035409708No Hit
GGCCAAGACTGTGCACCATCTTGAGTCCCTTTTTACCGCTGTTACCAATT8030.1602276309855895No Hit
ATACCACATCATCCATATAACTAAAAGCCAAACAGTGGGGGAAAGCCCTA7930.15823226820868302No Hit
CACGTCTCCTGCTCAAGGAACCTCTATGTATCCCTCCTGTTGCTGTACCA7930.15823226820868302No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTG7890.15743412309792043No Hit
GCATACAAGGGCTTTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGC7860.15683551426484849No Hit
ATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATAC7790.15543876032101395No Hit
GTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGTATGATGTGAT7780.1552392240433233No Hit
GTTCAGGAACAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCA7760.15484015148794203No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC7750.15464061521025138No Hit
GGACTCGTGCAGGTCCTGCAGGGTCCCGTGCTGGTGGTTGAAGATCCTGG7730.15424154265487008No Hit
ATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATGAA7670.1530443249887262No Hit
GCAAAACCCAAAAGACCCACAATACGTTGACAGACTTTCCAATCAATAGG7610.1518471073225823No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATACGTTGACAGACT7590.15144803476720103No Hit
GCCCATGACCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC7360.14685870038031615No Hit
ACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTT7340.14645962782493485No Hit
GACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCA7270.14506287388110034No Hit
CTTTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAA7140.1424689022711219No Hit
GGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG7090.14147122088266867No Hit
GGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACA6920.13807910416192767No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC6830.13628327766271187No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTGAA6710.13388884233042408No Hit
GTCTTGGCCCCCAATACCACATCATCCATATAACTAAAGGCCAAACAGTG6660.13289116094197087No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6610.13189347955351763No Hit
ATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTT6500.12969858049892052No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT6310.12590739122279823No Hit
CAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTG6310.12590739122279823No Hit
GCCCAAGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTT6310.12590739122279823No Hit
GGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC6300.12570785494510758No Hit
CTCCCATATCGTCAATCTTCTCGAGGATTGGGGACCTTGCGCTGAACATG6210.12391202844589173No Hit
CACTTGGCACAGACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGT6190.12351295589051045No Hit
ACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCGAGGATT6110.12191666566898528No Hit
GTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACAT5970.11912315778131621No Hit
CCAAGACTGTGCACCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTT5970.11912315778131621No Hit
TTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCATGG5950.11872408522593493No Hit
GTGCACCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC5930.11832501267055363No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA5920.11812547639286299No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA5910.11792594011517234No Hit
ATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATGAAATGTA5900.11772640383748169No Hit
GTGAAAGCCTGCTTAGATTGAATACATGCATACAAGGGCTTTAAAGCAGG5900.11772640383748169No Hit
GTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA5870.11712779500440976No Hit
ACCCAAAAGACCCACAATACGTTGACAGACTTTCCAATCAATAGGCCTGT5850.11672872244902846No Hit
ATGTGGTTATCCTGCTTTAAAGCCCTTGTATGCATGTATTCAATCTAAGC5780.11533196850519394No Hit
TACAAGGGCTTTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCAAA5770.11513243222750329No Hit
GTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTA5760.11493289594981264No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA5730.1143342871167407No Hit
GTGGTATTGGGGGCCAAAACTGTGCACCATCTTGAGTCCCTTTTTACCGC5730.1143342871167407No Hit
GTATTGGGGGCCAAAACTGTGCACCATCTTGAGTCCCTTTTTACCGCTGT5720.11413475083905004No Hit
ATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTCCT5680.11333660572828747No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAAACTGTGCACCATCTTGAG5680.11333660572828747No Hit
CGTCTGTCCTCTAATTCCAGGATCTTCAACCACCAGCACGGGACCCTGCA5650.11273799689521552No Hit
ACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTT5630.11233892433983424No Hit
TCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTT5530.11034356156292777No Hit
ATCTTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT5480.10934588017447452No Hit
TTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC5470.10914634389678389No Hit
GCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTA5430.1083481987860213No Hit
GGACAGGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCC5370.10715098111987739No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCCTGCAGGGTCCCGTG5350.1067519085644961No Hit
CATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGT5350.1067519085644961No Hit
GCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCTGGCC5290.10555469089835222No Hit
AGATCACATCATACAGAAAATCAAAGAATGTTTTAGGAAACTTCCTGTTA5290.10555469089835222No Hit
CCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT5190.10355932812144575No Hit
GGAGGCATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCCTGCAGG5170.10316025556606447No Hit
GCTTTAAAGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT5170.10316025556606447No Hit
GGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAACAAGAAGAT5170.10316025556606447No Hit
ACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC5160.10296071928837382No Hit
ATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5130.10236211045530187No Hit
GCAGAGGAGCCCGAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAG5120.10216257417761122No Hit
GACAGGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCA5040.10056628395608605No Hit
TGCTTAGATTGAATACATGCATACAAGGGCTTTAAAGCAGGATAACCACA5020.10016721140070477No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)