FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501162
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGT52361.0447719499882273No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG52271.0429761234890116No Hit
CCAAAACTGTGCACCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTT34250.6834117510904658No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT29640.5914255270750776No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23230.4635227730753728No Hit
CTGTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGC22450.44795894341550235No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC22410.44716079830473976No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT22060.4401770285855672No Hit
TACCTCGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG21570.43039975097872546No Hit
GACAAGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCA20760.414237312485783No Hit
CATCAAACAGAAAATCAAAGAATGTTTTAGGAAACTTCCTGTTAACAGGC20360.40625586137815717No Hit
CCTCTTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGT20150.4020655995466536No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC19430.38769898755292703No Hit
GGACTCGTGCAGGTTCTGCAGGGTCCCGTGCTGGTGGTTGAAGATCCTGG17760.35437642917858897No Hit
ATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGT17590.350984312457848No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA17020.3396107446294811No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT15450.3082835490320495No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGGAAACTTCCTGTTAACAGGC14960.2985062714252078No Hit
GGCCAAAACTGTGCACCATCTTGAGTCCCTTTTTACCGCTGTTACCAATT14720.2937174007606323No Hit
CTGTTTGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGC14690.29311879192756035No Hit
CCTCGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT14550.29032528403989133No Hit
GGTCCTGCAGGGTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGAC14470.28872899381836614No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG13830.2759586720461647No Hit
ACTGTGCACCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTT13800.27536006321309275No Hit
GAATACATGCATACAAGGGCTTTAAAGCAGGATAACCACATTGTGTAAAA13720.27376377299156757No Hit
GCACGTCTCCTGCTCAAGGAACCTCTATGTATCCCTCCTGTTGCTGTACC13460.26857582977161076No Hit
GGAGTCGTGCAGGTCCTGCAGGGTCCCGTGCTGGTGGTTGAAGATCCTGG13150.2623902051632007No Hit
GACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAG12790.25520689916633743No Hit
CTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCA12210.2436337950602799No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCTGTTGCTGTACC12040.2402416783395389No Hit
ATCGAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC11920.23784724300725113No Hit
ATGTGGTATTGGGGGCCAAAACTGTGCACCATCTTGAGTCCCTTTTTACC11860.23665002534110727No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAAAGCCCTTGTATGCATGTATT11850.23645048906341662No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA11790.23525327139727273No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT11590.2312625458434598No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT11490.22926718306655333No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA11450.22846903795579074No Hit
CATGCATACAAGGGCTTTAAAGCAGGATAACCACATTGTGTAAAAGGGGC11250.22447831240197783No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT11080.22108619568123683No Hit
ACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA10530.21011170040825122No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT10520.20991216413056057No Hit
CAGTCTTGGCCCCCAATACCACATCATCCATATAACTAAAGGCCAAACAG10510.20971262785286993No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG10500.20951309157517928No Hit
GATCCTGTGGCAATGACCCATAACATCCAATGACATAGCCCATGAAATGT10480.20911401901979798No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT10450.20851541018672604No Hit
GCCAAAACTGTGCACCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTT10370.20691911996520088No Hit
GTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACCTGTA10300.20552236602136634No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC10180.20312793068907858No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTG10090.20133210418986275No Hit
GTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCC9980.19913720513526564No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC9940.19833906002450305No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT9820.1959446246922153No Hit
ACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCAT9800.195545552136834No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCG9490.18935992752842393No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC9420.18796317358458942No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG9380.18716502847382682No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTCCTAAAAC9270.18497012941922972No Hit
GGAGACGTGCAGGTCCTGCAGGGTCCCGTGCTGGTGGTTGAAGATCCTGG8950.17858496853312902No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA8920.17798635970005708No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGGAAAATTCCTATGGGAG8880.17718821458929448No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA8740.1743947067016254No Hit
CAGTCTTGGCCCCCAATACCACATCATCCATATAACTAAAAGCCAAACAG8730.17419517042393476No Hit
ATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATAC8700.1735965615908628No Hit
GATTGAATACATGCATACAAGGGCTTTAAAGCAGGATAACCACATTGTGT8680.17319748903548154No Hit
GTTTTGGCCCCCAATACCACATCATCCATATAACTAAAAGCCAAACAGTG8600.17160119881395636No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA8590.1714016625362657No Hit
GTTCAGGAACAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCA8430.16820908209321536No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC8330.1662137193163089No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC8240.16441789281709307No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC8230.16421835653940242No Hit
GTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGTATGATGTGAT8180.1632206751509492No Hit
GTCTTGGCCCCCAATACCACATCATCCATATAACTAAAGGCCAAACAGTG8000.15962902215251756No Hit
TTCCCCCACTGTTTGGCTTTTAGTTATATGGATGATGTGGTATTGGGGGC7960.15883087704175497No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC7940.15843180448637367No Hit
GCTTAGATTGAATACATGCATACAAGGGCTTTAAAGCAGGATAACCACAT7740.15444107893256073No Hit
GGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACA7720.15404200637717944No Hit
ATCAAGGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATCTTCAACCACC7700.15364293382179814No Hit
GGCCAAGACTGTGCACCATCTTGAGTCCCTTTTTACCGCTGTTACCAATT7670.1530443249887262No Hit
ATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATGAA7660.15284478871103555No Hit
ATACCACATCATCCATATAACTAAAAGCCAAACAGTGGGGGAAAGCCCTA7510.14985174454567585No Hit
GGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG7460.1488540631572226No Hit
GGACTCGTGCAGGTCCTGCAGGGTCCCGTGCTGGTGGTTGAAGATCCTGG7370.1470582366580068No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATACGTTGACAGACT7330.1462600915472442No Hit
GACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCA7230.14426472877033775No Hit
CTTTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAA7140.1424689022711219No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA7120.14206982971574064No Hit
GCATACAAGGGCTTTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGC7070.14107214832728737No Hit
CACGTCTCCTGCTCAAGGAACCTCTATGTATCCCTCCTGTTGCTGTACCA6950.13867771299499962No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA6940.13847817671730897No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT6880.13728095905116508No Hit
CTCCCATATCGTCAATCTTCTCGAGGATTGGGGACCTTGCGCTGAACATG6880.13728095905116508No Hit
ATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTT6870.13708142277347443No Hit
GCAAAACCCAAAAGACCCACAATACGTTGACAGACTTTCCAATCAATAGG6830.13628327766271187No Hit
GTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACAT6720.13408837860811473No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA6630.13229255210889893No Hit
ACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTT6620.13209301583120828No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA6560.1308957981650644No Hit
ACCTCGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGA6470.12909997166584858No Hit
GCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTA6470.12909997166584858No Hit
TTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCATGG6400.12770321772201404No Hit
GGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC6230.12431110100127303No Hit
GCCCATGACCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC6220.12411156472358238No Hit
TCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTT6090.12151759311360398No Hit
TTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC6050.12071944800284139No Hit
CAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTG6030.1203203754474601No Hit
CAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTC6020.12012083916976946No Hit
ATGTGGTTATCCTGCTTTAAAGCCCTTGTATGCATGTATTCAATCTAAGC6010.11992130289207881No Hit
CATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGT5990.11952223033669751No Hit
GTATTGGGGGCCAAAACTGTGCACCATCTTGAGTCCCTTTTTACCGCTGT5940.11852454894824428No Hit
ACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCGAGGATT5900.11772640383748169No Hit
GCCCAAGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTT5850.11672872244902846No Hit
CACTTGGCACAGACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGT5780.11533196850519394No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAAACTGTGCACCATCTTGAG5740.11453382339443134No Hit
ACCCTGCGCTGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTG5680.11333660572828747No Hit
ATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTCCT5670.11313706945059682No Hit
ATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATGAAATGTA5670.11313706945059682No Hit
AGATCACATCATACAGAAAATCAAAGAATGTTTTAGGAAACTTCCTGTTA5650.11273799689521552No Hit
ATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5620.11213938806214359No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC5600.11174031550676229No Hit
ACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC5530.11034356156292777No Hit
GTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA5520.11014402528523712No Hit
ACCCAAAAGACCCACAATACGTTGACAGACTTTCCAATCAATAGGCCTGT5520.11014402528523712No Hit
GTGCACCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC5500.10974495272985582No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTGAA5470.10914634389678389No Hit
GCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCTGGCC5380.10735051739756804No Hit
CCAAGACTGTGCACCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTT5380.10735051739756804No Hit
GTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTC5370.10715098111987739No Hit
GTGAAAGCCTGCTTAGATTGAATACATGCATACAAGGGCTTTAAAGCAGG5370.10715098111987739No Hit
ACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTT5320.10615329973142416No Hit
GTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTA5290.10555469089835222No Hit
GCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAA5260.10495608206528029No Hit
CGTCTGTCCTCTAATTCCAGGATCTTCAACCACCAGCACGGGACCCTGCA5220.1041579369545177No Hit
CCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATC5210.10395840067682705No Hit
ATCTTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT5200.1037588643991364No Hit
CTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA5160.10296071928837382No Hit
GCTTTAAAGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT5110.10196303789992059No Hit
TCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTA5090.10156396534453929No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA5090.10156396534453929No Hit
GAACTACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC5070.101164892789158No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCT5030.1003667476783954No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)