FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505825
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCGTGTTCAGG47190.9329313497751198No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT33980.6717738348242969No Hit
TTTCTAAAACATTGCTTGAGTTTTTGTACAATATGTTCTTGTGGTAAAGT31320.6191864775366974No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG29460.5824148668017595No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23180.45826125636336684No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAA22650.44778332427222856No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT19760.39064893985073884No Hit
ATGATGCGCTGCGTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCAC18930.3742401028023526No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCG16640.3289675282953591No Hit
ATCCTAACCTTACCAAATATTTACCCTTGGATAAAGGCATTAAACCCTAT15050.2975337320219444No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT14900.2945682795433203No Hit
CTATATTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGCACT14080.2783571393268423No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCGTGTTCA13130.25957594029555675No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCG12920.2554243068254831No Hit
GTATTTGCCCTTGGATAAAGGCATTAAACCCTATTATCCTGAACACGCAG12440.24593485889388622No Hit
CCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC12240.24198092225572085No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12180.24079474126427122No Hit
CATTTGAACCCTAATAAAACCAAACGATGGGGTTACTCCCTTAACTTCAT12150.24020165076854644No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG12030.2378292887856472No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA11590.22913062818168342No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACACACT11540.22814214402214206No Hit
GTATACATGCGTATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA11430.22596747887115107No Hit
GTTGTTAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG11430.22596747887115107No Hit
ATCCTAACCTTACCAAGTATTTGCCCTTGGATAAAGGCATTAAACCCTAT11340.22418820738397668No Hit
ACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTGTA11290.22319972322443535No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC11040.21825730242672864No Hit
ATTTACCCTTGGATAAAGGCATTAAACCCTATTATCCTGAACACGCAGTT10900.21548954678001284No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCGTTAAC10720.211931003805664No Hit
GGGTAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAATTAATT10680.21114021647803094No Hit
GGTAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAATTAATTT10590.2093609449908565No Hit
ACACAAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTGTA10300.20362773686551672No Hit
CCCAAGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC10290.20343004003360848No Hit
GTTTTCGAAAACTGCCTGTAAATAGACCTATTGATTGGAAAGTGTGTCAA10220.20204616221025057No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT10080.19927840656353482No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10020.1980922255720852No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9830.19433598576582808No Hit
CTATTATCCTGAACACGCAGTTAATCATTACTTCAAAACTAGGCATTATT9700.19176592695102063No Hit
ATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA9470.18721889981713044No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG9250.18286956951514852No Hit
GACTTGTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT8950.17693866455790044No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8940.17674096772599218No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCC8530.16863539761775317No Hit
ATATTTACCCTTGGATAAAGGCATTAAACCCTATTATCCTGAACACGCAG8320.16448376414767954No Hit
AGGGCAAATACTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAATTAAT8280.16369297682004647No Hit
ATGATTAACTGCGTGTTCAGGATAATAGGGTTTAATGCCTTTATCCAAGG8260.16329758315622991No Hit
GCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAGAGCTACAGCAT8240.16290218949241336No Hit
ACCAAATATTTACCCTTGGATAAAGGCATTAAACCCTATTATCCTGAACA8130.16072752434142243No Hit
GTTCTATCCTAACCTTACCAAATATTTACCCTTGGATAAAGGCATTAAAC8080.1597390401818811No Hit
GTTTTGAAGTAATGATTAACTGCGTGTTCAGGATAATAGGGTTTAATGCC8050.15914594968615628No Hit
CCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCA7880.15578510354371572No Hit
AACTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT7830.15479661938417436No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7760.15341274156081647No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT7730.15281965106509168No Hit
GAAACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCC7690.1520288637374586No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA7660.1514357732417338No Hit
GGATAATAGGGTTTAATGCCTTTATCCAAGGGTAAATATTTGGTAAGGTT7590.15005189541837594No Hit
GGGTAAAGGTGCAGATATTGTTGACACAGAAAGGCCTTATAAGTTGGCGA7570.14965650175455938No Hit
CCAAATATTTACCCTTGGATAAAGGCATTAAACCCTATTATCCTGAACAC7390.14609795878021054No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACCG7340.14510947462066923No Hit
ACACTTGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA7290.14412099046112786No Hit
ATTCTTTGACACACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCT7250.1433302031334948No Hit
CATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAA7180.1419463253101369No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACCGTACCTGTC7170.14174862847822864No Hit
ATCTAGCCACCTGGGTGGGAGGTAATTTGGAAGATCCAGCATCCAGGGAA7170.14174862847822864No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAA7050.13937626649532942No Hit
ATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAG7040.13917856966342115No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG7000.13838778233578808No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA6890.13621311718479712No Hit
GTATACATTTGAACCCTAATAAAACCAAACGATGGGGTTACTCCCTTAAC6890.13621311718479712No Hit
GTTAAGGGAGTAACCCCATCGTTTGGTTTTATTAGGGTTCAAATGTATAC6830.1350269361933475No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC6820.13482923936143923No Hit
TAATAAAACCAAACGATGGGGTTACTCCCTTAACTTCATGGGATATGTAA6680.13206148371472348No Hit
AGTTAAGGGAGTAACCCCATCGTTTGGTTTTATTAGGGTTCAAATGTATA6670.13186378688281522No Hit
GGATAATAGGGTTTAATGCCTTTATCCAAGGGCAAATACTTGGTAAGGTT6600.13047990905945733No Hit
ATTAACAACAGTGCCAACAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6590.13028221222754904No Hit
ATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTT6450.1275144565808333No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA6410.12672366925320022No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA6350.1255374882617506No Hit
GCTTTATTCTTCTACCGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCT6270.12395591360648446No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCGTGTTCAGGAT6250.12356051994266792No Hit
GTTCTATCCTAACCTTACCAAGTATTTGCCCTTGGATAAAGGCATTAAAC6210.12276973261503485No Hit
CAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTAAT6190.12237433895121831No Hit
GCGTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTA6130.12118815795976869No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6120.12099046112786042No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG6090.12039737063213561No Hit
GGATAAAGGCATTAAACCCTATTATCCTGAACACGCAGTTAATCATTACT5970.11802500864923639No Hit
AGTCTAGACTCTTTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5920.11703652448969507No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGATGGGGTTACT5910.11683882765778679No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5810.1148618593387041No Hit
GGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG5800.11466416250679583No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGATGGGGTTACTCCCTTA5760.11387337517916275No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5730.11328028468343795No Hit
CTTACAGTTAACGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT5650.11169871002817179No Hit
ATACTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATC5630.11130331636435525No Hit
CTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGCACT5620.11110561953244699No Hit
CAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATT5610.11090792270053872No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATACGCATGTATA5570.11011713537290564No Hit
TAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGA5550.1097217417090891No Hit
CTACCGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC5540.10952404487718084No Hit
ACACGTGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA5540.10952404487718084No Hit
GTACAATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC5520.10912865121336432No Hit
AATACTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAAT5360.105965501902832No Hit
GGGTTACTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTT5310.10497701774329067No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT5310.10497701774329067No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5240.10359313991993278No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5240.10359313991993278No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5200.10280235259229971No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5200.10280235259229971No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAAC5190.10260465576039143No Hit
ATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTAATTA5160.10201156526466663No Hit
CTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCAC5120.10122077793703356No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCTAAAACATTGCTTG5120.10122077793703356No Hit
ACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTAA5100.10082538427321702No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACAGCCCCTGCTGGTGGCTCCA5080.10042999060940049No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5060.10003459694558395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)