FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505825
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCGTGTTCAGG50901.0062768744130874No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT33370.6597143280778925No Hit
TTTCTAAAACATTGCTTGAGTTTTTGTACAATATGTTCTTGTGGTAAAGT31100.6148371472347156No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG27540.5444570750753719No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAA24770.4896950526367815No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23070.4560865912123758No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT21620.42742055058567685No Hit
ATGATGCGCTGCGTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCAC20220.39974299411851927No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCG17830.352493451292443No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT14750.2916028270646963No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCGTGTTCA14180.280334107645925No Hit
CTATATTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGCACT13730.2714377502100529No Hit
GTATTTGCCCTTGGATAAAGGCATTAAACCCTATTATCCTGAACACGCAG12870.2544358226659418No Hit
ATCCTAACCTTACCAAATATTTACCCTTGGATAAAGGCATTAAACCCTAT12740.2518657638511343No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG12590.24890031137251029No Hit
GTTTTCGAAAACTGCCTGTAAATAGACCTATTGATTGGAAAGTGTGTCAA12500.24712103988533585No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCG12290.2429694064152622No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACACACT12240.24198092225572085No Hit
GTATACATGCGTATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA12040.23802698561755548No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA11940.23605001729847278No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11350.22438590421588492No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC11180.22102505807344436No Hit
GTTGTTAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG11070.21885039292245342No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT10900.21548954678001284No Hit
CATTTGAACCCTAATAAAACCAAACGATGGGGTTACTCCCTTAACTTCAT10680.21114021647803094No Hit
GGGTAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAATTAATT10580.20916324815894827No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCGTTAAC10340.2044185241931498No Hit
ATCCTAACCTTACCAAGTATTTGCCCTTGGATAAAGGCATTAAACCCTAT10290.20343004003360848No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10110.19987149705925963No Hit
GGTAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAATTAATTT10010.19789452874017693No Hit
ATTTACCCTTGGATAAAGGCATTAAACCCTATTATCCTGAACACGCAGTT9960.19690604458063563No Hit
ACACAAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTGTA9930.19631295408491078No Hit
CCCAAGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC9900.19571986358918597No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9820.19413828893391985No Hit
ACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTGTA9360.18504423466613948No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG9050.17891563287698314No Hit
CTATTATCCTGAACACGCAGTTAATCATTACTTCAAAACTAGGCATTATT8960.17713636138980873No Hit
GTTTTGAAGTAATGATTAACTGCGTGTTCAGGATAATAGGGTTTAATGCC8770.17338012158355162No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8460.1672515197943953No Hit
ATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8430.16665842929867047No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA8250.16309988632432165No Hit
GTTCTATCCTAACCTTACCAAATATTTACCCTTGGATAAAGGCATTAAAC8170.1615183116690555No Hit
AGGGCAAATACTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAATTAAT7800.15420352888844957No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACCGTACCTGTC7690.1520288637374586No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7690.1520288637374586No Hit
GCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAGAGCTACAGCAT7660.1514357732417338No Hit
GGATAATAGGGTTTAATGCCTTTATCCAAGGGTAAATATTTGGTAAGGTT7610.15044728908219246No Hit
ATGATTAACTGCGTGTTCAGGATAATAGGGTTTAATGCCTTTATCCAAGG7580.14985419858646765No Hit
GACTTGTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT7570.14965650175455938No Hit
AACTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT7490.14807492709929324No Hit
ATATTTACCCTTGGATAAAGGCATTAAACCCTATTATCCTGAACACGCAG7440.1470864429397519No Hit
CCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCA7340.14510947462066923No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCC7290.14412099046112786No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACCG7280.14392329362921957No Hit
GAAACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCC7280.14392329362921957No Hit
GGGTAAAGGTGCAGATATTGTTGACACAGAAAGGCCTTATAAGTTGGCGA7260.14352789996540305No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT7200.14234171897395342No Hit
ATCTAGCCACCTGGGTGGGAGGTAATTTGGAAGATCCAGCATCCAGGGAA7100.14036475065487075No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAA6990.1381900855038798No Hit
GTATACATTTGAACCCTAATAAAACCAAACGATGGGGTTACTCCCTTAAC6950.13739929817624671No Hit
ATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAG6930.1370039045124302No Hit
ACCAAATATTTACCCTTGGATAAAGGCATTAAACCCTATTATCCTGAACA6860.1356200266890723No Hit
ATTCTTTGACACACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCT6830.1350269361933475No Hit
GTTAAGGGAGTAACCCCATCGTTTGGTTTTATTAGGGTTCAAATGTATAC6810.13463154252953097No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG6800.13443384569762268No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6720.13285227104235653No Hit
ACACTTGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA6690.13225918054663174No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA6680.13206148371472348No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA6650.13146839321899867No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC6640.1312706963870904No Hit
GGATAATAGGGTTTAATGCCTTTATCCAAGGGCAAATACTTGGTAAGGTT6580.13008451539564078No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG6550.12949142489991597No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGATGGGGTTACT6530.12909603123609945No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA6510.1287006375722829No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCGTGTTCAGGAT6490.12830524390846637No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATACGCATGTATA6470.12790985024464982No Hit
CCAAATATTTACCCTTGGATAAAGGCATTAAACCCTATTATCCTGAACAC6340.12533979142984233No Hit
GCGTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTA6320.1249443977660258No Hit
GCTTTATTCTTCTACCGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCT6290.124351307270301No Hit
AGTTAAGGGAGTAACCCCATCGTTTGGTTTTATTAGGGTTCAAATGTATA6230.12316512627885139No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6220.12296742944694311No Hit
CCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC6190.12237433895121831No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6120.12099046112786042No Hit
GTTCTATCCTAACCTTACCAAGTATTTGCCCTTGGATAAAGGCATTAAAC6080.12019967380022735No Hit
GGATAAAGGCATTAAACCCTATTATCCTGAACACGCAGTTAATCATTACT6030.119211189640686No Hit
GTACAATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC6010.11881579597686946No Hit
GGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG6000.11861809914496121No Hit
ATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTT5980.11822270548114468No Hit
CATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAA5970.11802500864923639No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5880.11624573716206196No Hit
ATTAACAACAGTGCCAACAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5860.11585034349824543No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGATGGGGTTACTCCCTTA5770.11407107201107103No Hit
CAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTAAT5730.11328028468343795No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAAC5710.11288489101962142No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACAGCCCCTGCTGGTGGCTCCA5690.11248949735580488No Hit
GTCTATTTACAGGCAGTTTTCTAAAACATTGCTTGAGTTTTTGTACAATA5670.11209410369198834No Hit
CTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGCACT5590.11051252903672218No Hit
ATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTAATTA5520.10912865121336432No Hit
TAATAAAACCAAACGATGGGGTTACTCCCTTAACTTCATGGGATATGTAA5520.10912865121336432No Hit
CTACCGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC5500.10873325754954778No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGCGG5490.10853556071763949No Hit
AGTCTAGACTCTTTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5490.10853556071763949No Hit
GGATTAAAGACAGGTACGGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5420.1071516828942816No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5390.10655859239855682No Hit
TAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGA5390.10655859239855682No Hit
CTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCAC5350.10576780507092375No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT5280.10438392724756586No Hit
TTCTTCTACCGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5240.10359313991993278No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5210.10300004942420798No Hit
ACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTAA5090.10062768744130875No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT5060.10003459694558395No Hit
ATGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTAATTATG5060.10003459694558395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)