FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438738
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24410.5563684932693316No Hit
ATCCTAACCTTACCAAATATTTCCCCTTGGATAAAGGCATTAAACCTTAT19480.4440007475987947No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12260.2794378421746008No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG11440.260747872306479No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC9740.22200037379939735No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGT8860.20194284516043742No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGATGAGAGTCCAA8280.18872311037566838No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8160.18598799283399203No Hit
GTTCTATCCTAACCTTACCAAATATTTCCCCTTGGATAAAGGCATTAAAC8130.18530421344857295No Hit
GTATACATGCATATAAAGGCATTAAGGCCGGATAACCACATTGTGTAAAA8080.18416458113954115No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG7780.17732678728535026No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCATCAATGTCAACGACCGACCT7720.17595922851451207No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7460.17003314050754664No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7170.16342327311516214No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAGTCTCCTCC7100.1618277878825176No Hit
ATATGTTCCTGTGGTAAAGTACCCCAGCTTCCAATTACATATCCCATGAA7070.16114400849709848No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATTTCCTTTTTTCA7040.1604602291116794No Hit
CCCTTAATTTCATGGGATATGTAATTGGAAGCTGGGGTACTTTACCACAG7000.15954852326445396No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG6770.1543062146429076No Hit
CAATAGGCCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAAT6650.15157109710123126No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC6630.15111524417761854No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6410.14610086201787856No Hit
GGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA6390.14564500909426584No Hit
ATACTACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG6380.14541708263245945No Hit
ATGTAATTGGAAGCTGGGGTACTTTACCACAGGAACATATTGTATTAAAA5950.13561624477478587No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5950.13561624477478587No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC5920.1349324653893668No Hit
AGTCTAGACTCTGTAGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5880.13402075954214132No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT5830.1328811272331095No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5690.12969015676782045No Hit
GTATTCATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAAT5670.1292343038442077No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT5620.1280946715351759No Hit
CTGTAGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACC5610.1278667450733695No Hit
TCCACAGGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5580.12718296568795046No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5560.1267271127643377No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT5530.12604333337891863No Hit
GTATAAAGAATTTGGAGCCTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5450.12421992168446773No Hit
GGAACATATTGTATTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA5380.12262443645182318No Hit
TTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5330.12148480414279138No Hit
CTTTGGGACATGGACATTGACCCGTATAAAGAATTTGGAGCCTCTGTGGA5270.12011724537195319No Hit
TCCTAGTACAAAGATCATTAACCTAGTCTCCTCCCCCAACTCCTCCCAGT5180.11806590721569593No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTG5130.1169262749066641No Hit
CTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGACTA5120.11669834844485776No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5090.11601456905943866No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5050.1151028632122132No Hit
GTTGTCAATAGGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5000.1139632309031814No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4920.1121398192087305No Hit
GTACAAAGATCATTAACCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTA4900.11168396628511777No Hit
ACATTGACCCGTATAAAGAATTTGGAGCCTCTGTGGAGTTACTCTCTTTT4870.11100018689969868No Hit
CTTTTACACAATGTGGTTATCCGGCCTTAATGCCTTTATATGCATGTATA4790.10917677520524778No Hit
AATTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTCAAATGAATA4790.10917677520524778No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAGGTCTTGCCC4780.10894884874344142No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATG4760.10849299581982869No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC4660.10621373120176505No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC4660.10621373120176505No Hit
CTCATAAGGTGGGAAACTTTACTGGCCTTTATTCTTCTACTGTACCTGTC4660.10621373120176505No Hit
TTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGTTT4620.1053020253545396No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4610.10507409889273325No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4580.10439031950731416No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG4570.1041623930455078No Hit
ACATAAGAGGACTCTTGGACTCTCATCAATGTCAACGACCGACCTTGAGG4560.10393446658370144No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4520.103022760736476No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAATGAAAAA4440.10119934904202507No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC4440.10119934904202507No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAATACAATATGTT4430.10097142258021871No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)