FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438738
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24350.5550009344984934No Hit
ATCCTAACCTTACCAAATATTTCCCCTTGGATAAAGGCATTAAACCTTAT16180.368785015202695No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG13410.3056493852823325No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC13190.3006350031225925No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10320.2352201085841664No Hit
GTTCTATCCTAACCTTACCAAATATTTCCCCTTGGATAAAGGCATTAAAC10090.22997779996262008No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGT9560.21789769748688284No Hit
GTATACATGCATATAAAGGCATTAAGGCCGGATAACCACATTGTGTAAAA9150.20855271255282196No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGATGAGAGTCCAA8910.20308247746946925No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8680.1978401688479229No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCATCAATGTCAACGACCGACCT8620.19647261007708475No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG8450.19259786022637657No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7800.17778264020896298No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7220.16456290542419394No Hit
CCCTTAATTTCATGGGATATGTAATTGGAAGCTGGGGTACTTTACCACAG7120.1622836408061303No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATTTCCTTTTTTCA7100.1618277878825176No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6670.15202695002484398No Hit
GGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA6570.14974768540678035No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6550.14929183248316763No Hit
CTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGACTA6390.14564500909426584No Hit
ATATGTTCCTGTGGTAAAGTACCCCAGCTTCCAATTACATATCCCATGAA6320.1440495238616213No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG6310.14382159739981493No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAGTCTCCTCC6290.1433657444762022No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC6240.1422261121671704No Hit
CTTTGGGACATGGACATTGACCCGTATAAAGAATTTGGAGCCTCTGTGGA6220.14177025924355766No Hit
GTATAAAGAATTTGGAGCCTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6140.13994684754910675No Hit
CAATAGGCCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAAT6050.13789550939284947No Hit
GTATTCATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAAT5990.1365279506220113No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT5990.1365279506220113No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATG5870.13379283308033496No Hit
ATACTACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5790.13196942138588405No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5790.13196942138588405No Hit
ATGTAATTGGAAGCTGGGGTACTTTACCACAGGAACATATTGTATTAAAA5680.12946223030601406No Hit
CTTTTACACAATGTGGTTATCCGGCCTTAATGCCTTTATATGCATGTATA5570.12695503922614407No Hit
GTACAAAGATCATTAACCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTA5480.12490370106988681No Hit
TTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5450.12421992168446773No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5450.12421992168446773No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5410.12330821583724227No Hit
GTTGTCAATAGGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5280.12034517183375955No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5260.11988931891014684No Hit
GGAACATATTGTATTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA5220.11897761306292139No Hit
AGTCTAGACTCTGTAGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5160.1176100542920832No Hit
CTCATAAGGTGGGAAACTTTACTGGCCTTTATTCTTCTACTGTACCTGTC5080.1157866425976323No Hit
CTGTAGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACC5060.11533078967401957No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4980.11350737797956867No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4980.11350737797956867No Hit
TTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGTTT4970.11327945151776231No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4940.11259567213234321No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG4920.1121398192087305No Hit
TCCACAGGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA4860.11077226043789232No Hit
CTCTTGGACTCTCATCAATGTCAACGACCGACCTTGAGGCATACTTCAAA4840.11031640751427958No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT4710.10735336351079688No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4690.10689751058718415No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC4670.10644165766357143No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAGTCT4670.10644165766357143No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC4640.10575787827815233No Hit
TCCTAGTACAAAGATCATTAACCTAGTCTCCTCCCCCAACTCCTCCCAGT4630.10552995181634599No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4620.1053020253545396No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGGAAATATTTGGTAAGGTT4600.10484617243092689No Hit
ACATTGACCCGTATAAAGAATTTGGAGCCTCTGTGGAGTTACTCTCTTTT4540.1034786136600887No Hit
GTATACATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAAT4520.103022760736476No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT4510.10279483427466961No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC4460.1016552019656378No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4440.10119934904202507No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGCCTTTATTCTTCTACTG4440.10119934904202507No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT4440.10119934904202507No Hit
TTGACATACTTTCCAATCAATAGGCCTATTTACAGGCAGTTTCCGAAAAC4400.10028764319479963No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)