FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557089
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN51340.9215762651928148No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT16630.2985160360373298No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA13260.23802300889085945No Hit
TCCTCAGACCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG12730.22850926871648874No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC12330.22132908745281277No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA12080.21684147416301522No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT11330.20337863429362274No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGACACACCCGTG11220.20140408444611185No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11190.20086557085133616No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT11080.19889102100382525No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA11040.19817300287745765No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA10650.19117232614537352No Hit
GGAACATATTGTGCAAAAAATCAAAATGTGTTTTAGGAAACTGCCTGTAA9940.17842750440234864No Hit
GTCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT9150.16424664640658854No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT8850.1588615104588315No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGAAAGA8800.15796398780087204No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT8290.14880925668968514No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG7950.14270610261556052No Hit
CATTTAAACCCTCATAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7690.13803898479417112No Hit
GTTTACAGGCAGTTTCCTAAAACACATTTTGATTTTTTGCACAATATGTT7640.13714146213621164No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA7300.13103830806208702No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC7150.12834574008820854No Hit
AGTCTGTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT7100.12744821743024903No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC7090.12726871289865713No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT7070.12690970383547334No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6830.12260159507726771No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6750.12116555882453253No Hit
GTTGTGAGTAGGCCCTGGGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6740.12098605429294061No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC6420.11524190928199982No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6390.11470339568722411No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT6370.11434438662404033No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6040.10842073708150761No Hit
ACGTTAATATGGGCATAAAACTCAGACAACTATTGTGGTTTCACATTTCC5990.10752321442354812No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG5830.10465114191807773No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAATTGGCAAACTCCTT5780.10375361926011821No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5670.10177906941260732No Hit
GCTTACATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA5630.10106105128623973No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC5620.10088154675464782No Hit
CGTTAATATGGGCATAAAACTCAGACAACTATTGTGGTTTCACATTTCCT5620.10088154675464782No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG5620.10088154675464782No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)