FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558979
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN79121.4154377892550525No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG18370.3286348861048447No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT17290.3093139456043966No Hit
TTTTAGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACAT13870.24813096735297746No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA13510.2416906538528281No Hit
TTTTCTGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA13380.23936498508888526No Hit
GTACTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT13290.23775490671384794No Hit
CAATCCTTTGGCATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTC12350.220938532574569No Hit
GTACAGAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT12230.2187917614078525No Hit
ATCCTAACCTTACCAAATACTTGCCCTTGGATAAAGGCATTAAACCCTAT11690.20913129115762846No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC11020.19714515214346154No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT10850.19410389299061326No Hit
AACTGGAGGAGTGCGAATCCACACTCCAAAAGATACCAAATACTCAAGAA9930.17764531404578704No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT9440.16887933178169484No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9420.1685215365872421No Hit
AGCTTGAGGAGTGCGAATCCACACTCCAAAAGATACCAAATACTCAAGAA9070.16226012068431908No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8240.14741162011453024No Hit
CTCTTACAACTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8220.1470538249200775No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7790.13936122823934352No Hit
GTTTTATCCTAACCTTACCAAATACTTGCCCTTGGATAAAGGCATTAAAC7570.13542548110036334No Hit
GGATAATAGGGTTTAATGCCTTTATCCAAGGGCAAGTATTTGGTAAGGTT7440.13309981233642051No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7380.13202642675306228No Hit
CTTACAACTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT7340.1313108363641568No Hit
GACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACT7010.12540721565568652No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6890.12326044448897006No Hit
GTTGTAAGAGGGCCCACATATTGTTCACATCTATTAATAATGTCCTCCTG6770.1211136733222536No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6610.11825131176663166No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA6570.11753572137772617No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6560.1173568237804998No Hit
ACCAAATACTTGCCCTTGGATAAAGGCATTAAACCCTATTATCCTGAACA6540.11699902858604706No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6500.11628343819714158No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6480.11592564300268882No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6470.11574674540546245No Hit
ATGTTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACATTTCC6360.11377887183597238No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTTTCGCCAACTTACAAGGCC6350.113599974238746No Hit
CTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5980.10698076314137024No Hit
ATATGTGGGCCCTCTTACAACTAATGAAAAAAGGAGATTAAAATTAATTA5960.1066229679469175No Hit
GTAAACTGGAGGAGTGCGAATCCACACTCCAAAAGATACCAAATACTCAA5820.1041184015857483No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGGCATACT5790.1035817087940692No Hit
TTATTAATAGATGTGAACAATATGTGGGCCCTCTTACAACTAATGAAAAA5760.10304501600239006No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG5660.10125604003012635No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)