FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558979
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN79021.413648813282789No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG21320.38140967728662434No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT18240.3263092173409019No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA14100.252245612089184No Hit
TTTTAGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACAT12690.2270210508802656No Hit
TTTTCTGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA12490.2234430989357382No Hit
GTACAGAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT12290.21986514699121076No Hit
GTACTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT11780.2107413695326658No Hit
CAATCCTTTGGCATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTC11780.2107413695326658No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT11480.20537444161587465No Hit
ATCCTAACCTTACCAAATACTTGCCCTTGGATAAAGGCATTAAACCCTAT11400.2039432608380637No Hit
AGCTTGAGGAGTGCGAATCCACACTCCAAAAGATACCAAATACTCAAGAA10860.19428279058783962No Hit
AACTGGAGGAGTGCGAATCCACACTCCAAAAGATACCAAATACTCAAGAA9810.1754985428790706No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC9690.17335177171235414No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT8800.15742988555920706No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8760.15671429517030158No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8530.152599650434095No Hit
CTCTTACAACTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8330.1490216984895676No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7870.1407924090171545No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7830.140076818628249No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6980.12487052286400742No Hit
GGATAATAGGGTTTAATGCCTTTATCCAAGGGCAAGTATTTGGTAAGGTT6940.12415493247510193No Hit
CTTACAACTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT6780.12129257091947998No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6640.11878800455831078No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6620.11843020936385804No Hit
GTTTTATCCTAACCTTACCAAATACTTGCCCTTGGATAAAGGCATTAAAC6530.11682013098882069No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6410.11467335982210422No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA6400.11449446222487784No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6320.11306328144706687No Hit
GACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACT6250.11181099826648229No Hit
GTAAACTGGAGGAGTGCGAATCCACACTCCAAAAGATACCAAATACTCAA6220.11127430547480316No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6220.11127430547480316No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGGCATACT6110.10930643190531308No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5970.10680186554414388No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTTTCGCCAACTTACAAGGCC5910.10572847996078565No Hit
GTATTTGGTAAGGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATC5890.1053706847663329No Hit
GAACAATATGTGGGCCCTCTTACAACTAATGAAAAAAGGAGATTAAAATT5870.10501288957188017No Hit
GTTGTAAGAGGGCCCACATATTGTTCACATCTATTAATAATGTCCTCCTG5870.10501288957188017No Hit
TTATTAATAGATGTGAACAATATGTGGGCCCTCTTACAACTAATGAAAAA5840.10447619678020104No Hit
ATGTTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACATTTCC5760.10304501600239006No Hit
CTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5730.10250832321071096No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)