FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429595
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAGTAGTTGT37450.8717513006436294No Hit
GTCAACCATGTTAATGTTAATATGGGCCTAAAAATCAGACAACTACTGTG32020.7453531814848869No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCGGG30180.7025221429485912No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT28370.6603894365623436No Hit
TGGACAAAGGCATTAAACCATATTATCCCGAACATGCAGTTAATCATTAC23300.5423713032041807No Hit
CTCTTACAGTTAATGAAAAACGGAGATTAAAATTAATTATGCCTGCTAGG21100.49116027886730523No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT16390.38152213130972196No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16070.3740732550425401No Hit
ACAATTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAGTAGTTG15560.36220160849171895No Hit
CCTTTCTCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGG15250.3549855096078865No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCGTTTTTCA14260.3319405486562925No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA13590.3163444639718805No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT13240.3081972555546503No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13230.3079644781713009No Hit
GAGACACTGTTCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACT12810.29818782807062466No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10720.24953735495059298No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCG10020.23324293811613264No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA9940.23138071904933716No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA9550.22230240109870925No Hit
GAACAATTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAGTAGT9060.210896309314587No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8910.20740464856434546No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT8880.20670631641429718No Hit
CAACCATGTTAATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGT8630.20088688183056133No Hit
GCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACA8570.19949021753046473No Hit
CATAGCTGACTACTAATTCCCTGGATGCTGGGTCATCCAAATTACTTCCC8250.19204134126328287No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTATTAATAATATCCTCCTGT8240.19180856387993342No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCGTTTTTCATTAAC8190.19064467696318627No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGGCCCACAATTCTCTGACATACT7830.18226469116260663No Hit
ATATTATCCCGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT7810.18179913639590775No Hit
GGATATTATTAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATG7730.17993691732911232No Hit
CAGTGTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAGTAGTTGT7650.17807469826231684No Hit
TTATTAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAA7600.17691081134556966No Hit
GCCAATAGCACCTCCTCCTGCCTCCACCAATCGGCAGTCAGGAAGACAGC7560.17597970181217193No Hit
CAATATGCCCTGAGCCTGCGGGCTCCACCCCAAAAGACCGCCGTGTGGTG7500.17458303751207532No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG7490.1743502601287259No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG7300.16992748984508665No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAT7240.16853082554499005No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCATATTCATTTACAGG7070.16457361002804965No Hit
GGACAAAGGCATTAAACCATATTATCCCGAACATGCAGTTAATCATTACT7060.16434083264470023No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6990.1627113909612542No Hit
CCTCCTGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA6970.16224583619455535No Hit
CTCGAAAATACACCTCCTTTCCATGGCTGCTAGGGTGTGCTGCCAACTGG6940.16154750404450705No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6800.15828862067761498No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA6770.15759028852756668No Hit
CCCTTAACTTCATGGGATATGTAATTGGATGTTGGGGTACTTTACCGCAA6730.15665917899416892No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGAGAAAGGCATTAAACCATAT6440.14990863487703535No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6440.14990863487703535No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6180.14385642290995007No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA6090.14176142645980516No Hit
TATTAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAAC6000.13966643000966028No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA5960.13873532047626252No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGTACAATA5840.1359419918760693No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5740.13361421804257498No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5650.13151922159243007No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC5610.13058811205903234No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCGGGATAATATGGTTTAATGCC5600.13035533467568292No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5600.13035533467568292No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA5560.1294242251422852No Hit
GCCTTAGAGTCTCCGGAACATTGTTCTCCTCACCATACAGCACTCAGGCA5530.12872589299223688No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5510.128260338225538No Hit
ATTCGAGATCTCCTCGACACCGCCTCAGCTCTGTATCGGGAGGCCTTAGA5500.12802756084218858No Hit
ATGTAATTGGATGTTGGGGTACTTTACCGCAAGAACATATTGTACAAAAA5480.1275620060754897No Hit
GCTGAGAGTCCAAGAGTCCTCTTATATAAGACCTTGGGCAAGACCTGGTG5380.12523423224199537No Hit
CTATTAATAATATCCTCCTGTAAATGAATATGAGGAAAGGAGGGAGTTTG5350.12453590009194705No Hit
GGGAATTAGTAGTCAGCTATGTTAATGTTAATATGGGCCTAAAAATCAGA5270.12267368102515161No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA5260.12244090364180217No Hit
GGGCACATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATC5260.12244090364180217No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5210.12127701672505499No Hit
AACAATTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAGTAGTT5200.12104423934170556No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5150.11988035242495838No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT5150.11988035242495838No Hit
GTATAGGCCCCTCACTCTGGGGTCTAGCAGAGTTTGGTGGAATGTTGTGG5080.11825091074151234No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGTACAAT5050.11755257859146406No Hit
ATGTTAATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCAC5020.11685424644141576No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5010.11662146905806632No Hit
CTTTCTCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGC4980.11592313690801802No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT4970.11569035952466858No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC4960.11545758214131915No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT4960.11545758214131915No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG4930.11475924999127085No Hit
GACACATCCAGCGATAGCCAGGACAAATTGGAGGACAAGAGGTTGGTGAG4890.11382814045787311No Hit
CTCCAAAAGACACCAAATACTCAAGAACAATTTCTCTTCCAAAAGTAAGA4870.11336258569117424No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG4770.11103481185767991No Hit
ATTATTAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAAAA4650.1082414832574867No Hit
TGTCAGTTCCGATAAGTTTCGCTCCAGACCGGCTGCGAGCAAAACAAGCT4620.1075431511074384No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4570.10637926419069124No Hit
ACAGTGTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAGTAGTTG4570.10637926419069124No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGACCCTCTATG4560.1061464868073418No Hit
GGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGATGAC4560.1061464868073418No Hit
GTCAGCTATGTTAACGTTAATATGGGCCTAAAAATCAGACAACTACTGTG4550.10591370942399238No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4480.10428426774054633No Hit
GCTTAGATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT4470.1040514903571969No Hit
CTTACAGTTAATGAAAAACGGAGATTAAAATTAATTATGCCTGCTAGGTT4450.10358593559049803No Hit
GGGAATTAGTAGTCAACCATGTTAATGTTAATATGGGCCTAAAAATCAGA4440.10335315820714859No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAAC4390.10218927129040142No Hit
GCGTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTA4350.10125816175700368No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4330.10079260699030483No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)