FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429595
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAGTAGTTGT37050.8624402053096522No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCGGG32110.7474481779350318No Hit
GTCAACCATGTTAATGTTAATATGGGCCTAAAAATCAGACAACTACTGTG30140.7015910334151934No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT28030.6524750055284628No Hit
CTCTTACAGTTAATGAAAAACGGAGATTAAAATTAATTATGCCTGCTAGG20240.471141423899254No Hit
TGGACAAAGGCATTAAACCATATTATCCCGAACATGCAGTTAATCATTAC19310.44949312724775664No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT18300.4259826115294638No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16020.3729093681257929No Hit
CCTTTCTCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGG14700.3421827535236676No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA14090.3279833331393522No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCGTTTTTCA13830.3219311211722669No Hit
ACAATTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAGTAGTTG13660.3179739056553265No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT13130.30563670433780654No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12420.28910951011999675No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11650.27118565160209035No Hit
GAGACACTGTTCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACT11180.2602451145846669No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA9960.23184627381603604No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCG9890.23021683213259003No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9320.21694852128167227No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA9150.2129913057647319No Hit
GAACAATTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAGTAGT8940.2081029807143938No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGGCCCACAATTCTCTGACATACT8490.19762799846366924No Hit
GCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACA8450.1966968889302715No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT8320.19367078294672888No Hit
CAGTGTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAGTAGTTGT8250.19204134126328287No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCGTTTTTCATTAAC8010.18645468406289645No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAT7930.184592464996101No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTATTAATAATATCCTCCTGT7830.18226469116260663No Hit
CATAGCTGACTACTAATTCCCTGGATGCTGGGTCATCCAAATTACTTCCC7660.17830747564566626No Hit
TTATTAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAA7590.17667803396222023No Hit
GGATATTATTAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATG7590.17667803396222023No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCATATTCATTTACAGG7540.17551414704547308No Hit
CAACCATGTTAATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGT7480.17411748274537647No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG7460.1736519279786776No Hit
ATATTATCCCGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT7400.172255263678581No Hit
CCCTTAACTTCATGGGATATGTAATTGGATGTTGGGGTACTTTACCGCAA7290.16969471246173723No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7230.16829804816164062No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG7150.16643582909484514No Hit
CTCGAAAATACACCTCCTTTCCATGGCTGCTAGGGTGTGCTGCCAACTGG7070.16457361002804965No Hit
CAATATGCCCTGAGCCTGCGGGCTCCACCCCAAAAGACCGCCGTGTGGTG7060.16434083264470023No Hit
CCTCCTGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA6810.15852139806096438No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA6810.15852139806096438No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA6480.15083974441043307No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6450.15014141226038477No Hit
GGACAAAGGCATTAAACCATATTATCCCGAACATGCAGTTAATCATTACT6450.15014141226038477No Hit
GCCAATAGCACCTCCTCCTGCCTCCACCAATCGGCAGTCAGGAAGACAGC6210.14455475505999837No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6130.1426925359932029No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6050.14083031692640743No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCGGGATAATATGGTTTAATGCC5990.13943365262631083No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA5970.13896809785961195No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC5910.13757143355951534No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5870.13664032402611762No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGTACAATA5830.1357092144927199No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGAGAAAGGCATTAAACCATAT5670.13198477635912895No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5620.13082088944238177No Hit
TATTAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAAC5620.13082088944238177No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG5590.13012255729233346No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5570.1296570025256346No Hit
GCCTTAGAGTCTCCGGAACATTGTTCTCCTCACCATACAGCACTCAGGCA5570.1296570025256346No Hit
ATGTAATTGGATGTTGGGGTACTTTACCGCAAGAACATATTGTACAAAAA5560.1294242251422852No Hit
GCGTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTA5490.12779478345883916No Hit
CTCCAAAAGACACCAAATACTCAAGAACAATTTCTCTTCCAAAAGTAAGA5470.12732922869214028No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5430.12639811915874252No Hit
ATTCGAGATCTCCTCGACACCGCCTCAGCTCTGTATCGGGAGGCCTTAGA5420.1261653417753931No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5380.12523423224199537No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGTACAAT5380.12523423224199537No Hit
ATGTTAATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCAC5310.12360479055854934No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5170.12034590719165726No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA5090.11848368812486178No Hit
GTATAGGCCCCTCACTCTGGGGTCTAGCAGAGTTTGGTGGAATGTTGTGG5050.11755257859146406No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC4980.11592313690801802No Hit
GGGAATTAGTAGTCAGCTATGTTAATGTTAATATGGGCCTAAAAATCAGA4980.11592313690801802No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4970.11569035952466858No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG4880.11359536307452367No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGACCCTCTATG4840.11266425354112594No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAAC4800.11173314400772821No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT4800.11173314400772821No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT4800.11173314400772821No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4780.11126758924102935No Hit
GTCAGCTATGTTAACGTTAATATGGGCCTAAAAATCAGACAACTACTGTG4760.11080203447433049No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4750.11056925709098105No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGT4720.10987092494093274No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4700.10940537017423387No Hit
CTTTCTCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGC4670.10870703802418556No Hit
AACAATTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAGTAGTT4610.10731037372408896No Hit
CTATTAATAATATCCTCCTGTAAATGAATATGAGGAAAGGAGGGAGTTTG4600.10707759634073953No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4540.10568093204064294No Hit
TGTCAGTTCCGATAAGTTTCGCTCCAGACCGGCTGCGAGCAAAACAAGCT4520.10521537727394407No Hit
GGGCACATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATC4510.10498259989059464No Hit
GGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGATGAC4370.10172371652370255No Hit
GCTTAGATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT4330.10079260699030483No Hit
GAATTAGTAGTCAGCTATGTTAATGTTAATATGGGCCTAAAAATCAGACA4320.10055982960695539No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)