FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492035
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35510.7216966272724501No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCATGTTCAGG21790.44285467497230885No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT17860.36298230816913435No Hit
ATCCTAACCTTACCAAGTATTTGCCCTTGGATAAAGGCATTAAACCTTAT17120.3479427276514882No Hit
CTCTTACAGTTAATGAAAAGAGGAGATTAAAATTAATTATGCCTGCTAGG13380.2719318747650066No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAG11910.24205595130427715No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC11620.23616206164195636No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA10750.21848039265499405No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTCTTTTCA10550.21441564116373835No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9380.1906368449398925No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAAGAGGGAGTTTG9210.18718180617232513No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC9080.18453971770300892No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9030.18352352983019501No Hit
GCACTGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG9030.18352352983019501No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8950.1818976292336927No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8790.17864582804068815No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC8720.17722316501874869No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA8650.17580050199680916No Hit
GTTCTATCCTAACCTTACCAAGTATTTGCCCTTGGATAAAGGCATTAAAC8520.17315841352749298No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC8170.16604509841779547No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCTTTTCCTAAC8160.16584186084323269No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8060.16380948509760485No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7970.1619803469265398No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCAT7960.16177710935197698No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7900.1605576839046003No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA7810.15872854573353523No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATACTTGGTAAGGTT7700.15649293241334458No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7490.1522249433475261No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7480.1520217057729633No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAGAGACCCACAATTCTTTGACATACT7430.15100551790014938No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA7240.14714400398345645No Hit
GGAACATATTGTACTAAAAATTAAGCAATGTTTTCGAAAACTGCCTGTAA7220.1467375288343309No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCA6940.1410468767465729No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA6920.14064040159744734No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTCTTTTCATTAAC6870.1396242137246334No Hit
TAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAA6630.13474651193512655No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6600.13413679921143823No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCATGTTCA6540.13291737376406149No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCC6530.1327141361894987No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6450.13108823559299643No Hit
GTCCAGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6400.13007204772018252No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCTTTTCCTAACATTCATTTACAGG6400.13007204772018252No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6150.12499110835611288No Hit
AAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAA5950.12092635686485717No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC5920.12031664414116881No Hit
CTATATAAGAGAGAAACTACACGCAGTGCCTCATTTTGTGGGTCACCATA5880.11950369384291769No Hit
GGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGA5480.11137419086040627No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5430.11035800298759234No Hit
CTTATAAGGCCTTTCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCC5420.11015476541302956No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA5410.10995152783846676No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATACTTGGTA5410.10995152783846676No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTAATTTTTAGTACAATA5410.10995152783846676No Hit
ATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGAGGCAC5380.10934181511477843No Hit
ATTGACAACAGCGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT5340.10852886481652728No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCTTTTC5340.10852886481652728No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5330.10832562724196448No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG5250.1066997266454622No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5240.10649648907089942No Hit
GTTTGGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC5120.104057638176146No Hit
GAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA5090.10344792545245766No Hit
CAATTGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAG5060.1028382127287693No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5020.10202526243051815No Hit
AGTTAAGGGAGTAGCCCCAACGCTTGGTTTTATTAGGGTTCAAATGTATA4990.10141554970682981No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT4940.10039936183401588No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)