FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492035
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35270.7168189254829432No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCATGTTCAGG26700.5426443240826364No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT17210.34977186582255326No Hit
ATCCTAACCTTACCAAGTATTTGCCCTTGGATAAAGGCATTAAACCTTAT15010.3050595994187405No Hit
CTCTTACAGTTAATGAAAAGAGGAGATTAAAATTAATTATGCCTGCTAGG13150.26725741055006247No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAG13100.2662412226772486No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTCTTTTCA10580.21502535388742672No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA10280.20892822665054314No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAAGAGGGAGTTTG9620.1955145467293993No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA9520.1934821709837715No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9490.19287245826008312No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9060.18413324255388336No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8950.1818976292336927No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8890.18067820378631602No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATACTTGGTAAGGTT8450.17173575050555345No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTAATTTTTAGTACAATA8310.1688904244616745No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCTTTTCCTAAC8150.1656386232686699No Hit
GCACTGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG8140.16543538569410712No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA8110.16482567297041878No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC8030.1631997723739165No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAGAGACCCACAATTCTTTGACATACT7970.1619803469265398No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7950.1615738717774142No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7930.16116739662828866No Hit
GTTCTATCCTAACCTTACCAAGTATTTGCCCTTGGATAAAGGCATTAAAC7890.1603544463300375No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCATGTTCA7660.15567998211509343No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC7440.15120875547471216No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA7400.15039580517646103No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA7240.14714400398345645No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7220.1467375288343309No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7190.14612781611064254No Hit
GTCCAGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC7130.14490839066326583No Hit
GGAACATATTGTACTAAAAATTAAGCAATGTTTTCGAAAACTGCCTGTAA6940.1410468767465729No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCAT6860.13942097615007062No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6730.1367788876807544No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC6630.13474651193512655No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCA6460.1312914731675592No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTCTTTTCATTAAC6330.128649384698243No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCTTTTCCTAACATTCATTTACAGG6200.1260072962289268No Hit
AAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAA6160.12519434593067566No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC6160.12519434593067566No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6110.12417815805786175No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6060.1231619701850478No Hit
TAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAA5970.12133283201398275No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5730.1164551302244759No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG5730.1164551302244759No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5670.1152357047770992No Hit
CTTATAAGGCCTTTCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCC5550.11279685388234577No Hit
GTTTGGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC5500.11178066600953185No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5430.11035800298759234No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA5380.10934181511477843No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5260.106902964220025No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCC5200.1056835387726483No Hit
ATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGAGGCAC5190.1054803011980855No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCTTTTC5120.104057638176146No Hit
CTATATAAGAGAGAAACTACACGCAGTGCCTCATTTTGTGGGTCACCATA5090.10344792545245766No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATACTTGGTA5040.10243173757964372No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGACGGGATGGGAATACAAG5040.10243173757964372No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCCAAACTAGGCATTATT4960.10080583698314144No Hit
GTATTTGCCCTTGGATAAAGGCATTAAACCTTATTATCCTGAACATGCAG4940.10039936183401588No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)