FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287950
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG9050.31429067546449035No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT8850.3073450251779823No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8570.297621114776871No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT8470.29414828963361694No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG7700.26740753603056083No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT7650.26567112345893384No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7230.2510852578572669No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT6850.23788852231290156No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6800.2361521097412745No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC6720.23337384962667132No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA6640.2305955895120681No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT6340.22017711408230597No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA6260.21739885396770275No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA6250.21705157145337733No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG6240.21670428893905191No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA5890.2045494009376628No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT5880.2042021184233374No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC5780.20072929328008335No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA5720.19864559819413094No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT5530.19204723042194827No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG5360.18614342767841638No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA5330.18510158013544017No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC5270.18301788504948777No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG5160.17919777739190831No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG5150.17885049487758292No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG5020.17433582219135266No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA5000.17364125716270187No Hit
GTCTAATGGTAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA4990.17329397464837645No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC4440.15419343636047925No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4350.1510678937315506No Hit
CACTAAATATTTACCATTAGACAAAGGAATTAAACCTTATTATCCAGATC4310.149678763674249No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT4300.1493314811599236No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT4290.1489841986455982No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTC4260.147942351102622No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT4210.14620593853099498No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA4200.14585865601666956No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA4190.14551137350234414No Hit
CCATTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA4180.14516409098801877No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAATGGTAAATATTTAGTGTGGGT4180.14516409098801877No Hit
GGGTAAAGGTTCAGGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4140.14377496093071715No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC3980.13821844070151068No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG3950.13717659315853448No Hit
ATTCTATCCTACCCACACTAAATATTTACCATTAGACAAAGGAATTAAAC3810.13231463795797882No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG3780.1312727904150026No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3750.13023094287202638No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA3720.12918909532905018No Hit
GTATAAAGGGACTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC3680.12779996527174856No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC3620.12571627018579615No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTT3580.12432714012849454No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG3570.12397985761416913No Hit
GGTAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTT3550.1232852925855183No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC3510.12189616252821671No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA3470.1205070324709151No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA3430.11911790241361349No Hit
TTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTC3420.11877061989928807No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT3320.11529779475603405No Hit
GTCTAGGAGATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA3310.11495051224170863No Hit
TCCTCAGGCCATGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGG3270.11356138218440702No Hit
CCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCT3220.11182496961277999No Hit
CTATTGTGGTTTCATATATCTTGCCTTACTTTTGGAAGAGAGACTGTACT3220.11182496961277999No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT3210.1114776870984546No Hit
GTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCA3180.11043583955547838No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG3160.10974127452682758No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT3160.10974127452682758No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA3160.10974127452682758No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACAG3140.10904670949817676No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC3130.10869942698385138No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACAGTACCTATC3130.10869942698385138No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTCAAGTA3120.10835214446952596No Hit
GTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA3110.10800486195520055No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3070.10661573189789894No Hit
CAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAACCTCCGCG3050.10592116686924814No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT3050.10592116686924814No Hit
GTATCGAGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTG3000.10418475429762111No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT2970.10314290675464491No Hit
ATCCTACCCACACTAAATATTTACCATTAGACAAAGGAATTAAACCTTAT2940.1021010592116687No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG2920.10140649418301788No Hit
CTCTAAGGCTTCTCGATACAGAGCTGAGGCGGTGTCTAGGAGATCTCTGA2900.10071192915436708No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT2900.10071192915436708No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAAC2890.10036464664004167No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT2880.10001736412571627No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)