FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561963
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36790.6546694355322326No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCAGG31620.5626704961002771No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCGTAT25670.45679163930721417No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA23890.42511695609853317No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT18770.3340077549589564No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG17980.31994988993937323No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA16870.3001976998485666No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA15210.2706583885415944No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA13190.23471296152949572No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG12740.2267053168980876No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11390.20268238300386326No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC11260.20036906344367866No Hit
GTATACATGCATATAAAGGCATCAATGCAGGATAGCCACATTGTGTAAAA10770.19164962817836761No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT10540.18755683203342569No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10440.18577735544866833No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA10430.1855994077901926No Hit
GTTTTGAAGTAATGATTAACTGCATTTTCAGGATAATACGGTTTAATGCC9850.1752784435985999No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA9850.1752784435985999No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9740.1733210193553668No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG9720.17296512403841532No Hit
ATTTACAGGCAGTTTGCGAAAACATTGCTTGATTTTTTGTACAATATGTT9350.16638106067481312No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCA9240.16442363643158No Hit
GCCTAGTTTTGAAGTAATGATTAACTGCATTTTCAGGATAATACGGTTTA9040.1608646832620653No Hit
TTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8920.15872931136035648No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC8790.1564159918001719No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA8450.15036577141199686No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTGCGAAAAC8400.14947603311961818No Hit
GGATAATACGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT8380.14912013780266672No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC8350.1485862948272395No Hit
CATATAAAGGCATCAATGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA8290.1475186088763851No Hit
GTCTATTTACAGGCAGTTTGCGAAAACATTGCTTGATTTTTTGTACAATA8170.14538323697467626No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8010.1425360744390645No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC7740.13773148766021961No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7610.13541816810003507No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7540.1341725344907049No Hit
CAATAGGTCTATTTACAGGCAGTTTGCGAAAACATTGCTTGATTTTTTGT7460.132748953222899No Hit
ATGTAAGACCTTGGGCAACACCTGGCGGGCGTTCACGGTGGTCTCCATGC7440.13239305790594755No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7420.1320371625889961No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7420.1320371625889961No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA7360.13096947663814165No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7280.12954589537033576No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAATGCAGGATAGCCACAT7210.12830026176100562No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7170.12758847112710267No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7050.12545309922539385No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAAGAACATATTGTACAAAAA7030.12509720390844237No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6860.12207209371435486No Hit
ATACGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGAT6840.12171619839740339No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA6780.12064851244654898No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6770.12047056478807323No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6770.12047056478807323No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6630.11797929756941293No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT6590.11726750693551No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT6550.11655571630160705No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6520.11602187332617984No Hit
GGTCTATTTACAGGCAGTTTGCGAAAACATTGCTTGATTTTTTGTACAAT6490.11548803035075263No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6490.11548803035075263No Hit
ATATAGAGCTGAAGCGGTGTCGAGGAGATCTCGAATAGAAGGAAAGAAAT6480.1153100826922769No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA6230.1108613912303835No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6090.10837012401172319No Hit
ATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6010.1069465427439173No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA5910.10516706615915994No Hit
AGAACATATTGTACAAAAAATCAAGCAATGTTTTCGCAAACTGCCTGTAA5900.10498911850068421No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG5890.10481117084220846No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT5880.10463322318373273No Hit
CTTTTACACAATGTGGCTATCCTGCATTGATGCCTTTATATGCATGTATA5860.10427732786678126No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATTCG5780.10285374659897537No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5750.10231990362354816No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT5740.10214195596507245No Hit
ATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG5700.10143016533116948No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5700.10143016533116948No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5680.10107427001421801No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTGCG5630.10018453172183933No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)