FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619782
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30800.4969489272034361No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG20040.3233394967908071No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT19730.3183377381079154No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG18270.2947810681820382No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCGGAGAGTCCAA16550.26702937484470346No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA16160.2607368397275171No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15300.24686099305884973No Hit
GGATATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG14720.23750286391021358No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCTACAATTCTTTGACATACT14420.2326624522816087No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT13450.2170117880157862No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA13020.2100738646814525No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12990.20958982351859204No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCAT12950.2089444353014447No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT12130.19571397684992464No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCTATAA11820.19071221816703293No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTGTTGATAATATCCTCTTTC11730.18926009467845145No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT11210.1808700478555363No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA10850.17506155390121042No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC10390.16763958940401624No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTAGGTCT10370.16731689529544258No Hit
ATTCTATGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA10040.1619924425039772No Hit
ATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAA10000.16134705428682988No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC9850.15892684847252744No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9600.1548931721153567No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTT9570.1544091309524962No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC9500.15327970157248838No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA9420.15198892513819376No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC9270.1495687193238913No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCA9150.14763255467244935No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTA9010.1453736959124337No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC9000.1452123488581469No Hit
CTCCAAAAGACACCAAGTACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA8870.1431148371524181No Hit
GCACTAAGGCAAGCTATTCTATGTTGGGGTGAGTTGATGAATCTGGCCAC8560.1381130784695264No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA8540.13779038436095273No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTG8510.13730634319809223No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8320.13424074916664247No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACAACTATT8310.13407940211235564No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAAT8300.1339180550580688No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG8250.13311131978663465No Hit
CTTATGGCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACA8160.1316591962980532No Hit
ATGTTAACATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC8160.1316591962980532No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7980.12875494932089027No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA7960.12843225521231658No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC7890.12730282583230879No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7870.12698013172373512No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG7820.12617339645230097No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC7730.1247212729637195No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT7720.12455992590943267No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG7690.12407588474657218No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGATGATATGG7620.12294645536656437No Hit
GTATAGGCCCCTCACTCTGGGATCGAGCAGAGCTTGGTGGAATGTGCTGG7610.12278510831227754No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA7600.12262376125799072No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC7490.1208489436608356No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC7470.12052624955226192No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG7470.12052624955226192No Hit
ATCCCAGAGTGAGGGGCCTATACTTTCCTGCTGGTGGCTCAAGTTCCGGA7440.12004220838940144No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTAGGTCTTCTGG7390.11923547311796728No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACA7320.11810604373795948No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7320.11810604373795948No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG7240.11681526730366483No Hit
TTATTATCCTGAACATACAGTTAATCATTACTTCCAAACTAGACATTATT7200.1161698790865175No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA7170.11568583792365703No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7130.1150404497065097No Hit
CCAAAAGACACCAAGTACTCTAGAACAGTTTCTCTTCCAAAAGTAAGACA7060.1139110203265019No Hit
CTCTGGGATCGAGCAGAGCTTGGTGGAATGTGCTGGAGTTCCACTGCATG7030.1134269791636414No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6970.11245889683792043No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6890.1111681204036258No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6880.11100677334933896No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6800.10971599691504431No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT6730.10858656753503651No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6710.10826387342646286No Hit
GATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGT6710.10826387342646286No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGACAACGGTCAGGTC6680.10777983226360237No Hit
CCTTTACCCCGTTGCTCGACAACGGTCAGGTCTTTGCCAAGTGTTTGCTG6650.10729579110074189No Hit
GTTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCC6650.10729579110074189No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGTC6520.10519827939501308No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTATAGGAAGTTTCCGAAAAC6500.10487558528643944No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6470.10439154412357893No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTCG6450.10406885001500528No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6440.10390750296071845No Hit
GATTAACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC6380.10293942063499747No Hit
GGCCATAAGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGATCCATAC6350.10245537947213698No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)