FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619782
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30650.49452872138913356No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG22020.35528621353959944No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT17460.281711956784805No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG16960.2736446040704635No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA16890.27251517469045566No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCTACAATTCTTTGACATACT15690.2531535281760361No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCGGAGAGTCCAA14800.23879364034450823No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13800.22265893491582525No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG13160.2123327234414681No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA12950.2089444353014447No Hit
GGATATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG12610.20345863545569248No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT12200.1968434062299325No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCAT11930.19248703576418805No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTGTTGATAATATCCTCTTTC11530.18603315359271486No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA11170.18022465963838896No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT11060.17844984204123385No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCTATAA10130.1634445659925587No Hit
ATTCTATGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA10130.1634445659925587No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC9760.15747472498394596No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTAGGTCT9670.15602260149536448No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC9660.15586125444107768No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT9660.15586125444107768No Hit
CTCCAAAAGACACCAAGTACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA9420.15198892513819376No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC9310.15021410754103862No Hit
ATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAA9210.14860063699817033No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA9030.1456963900210074No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG8900.1435988783152786No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8870.1431148371524181No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTG8600.13875846668667371No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8600.13875846668667371No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTT8580.13843577257810002No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCA8510.13730634319809223No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC8500.1371449961438054No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA8450.13633826087237125No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTA8420.13585421970951078No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAAT8390.13537017854665026No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGATGATATGG8310.13407940211235564No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACAACTATT8230.132788625678061No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC8000.1290776434294639No Hit
CTTATGGCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACA7900.12746417288659562No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC7890.12730282583230879No Hit
GCACTAAGGCAAGCTATTCTATGTTGGGGTGAGTTGATGAATCTGGCCAC7860.1268187846694483No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACA7700.12423723180085902No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7700.12423723180085902No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT7670.12375319063799853No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC7550.12181702598655657No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA7470.12052624955226192No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT7430.11988086133511461No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7340.11842873784653314No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGACCGACCACG7320.11810604373795948No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC7310.11794469668367263No Hit
GTATAGGCCCCTCACTCTGGGATCGAGCAGAGCTTGGTGGAATGTGCTGG7260.1171379614122385No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7100.11455640854364922No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG7040.11358832621792825No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG7040.11358832621792825No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG7040.11358832621792825No Hit
ATCCCAGAGTGAGGGGCCTATACTTTCCTGCTGGTGGCTCAAGTTCCGGA7010.11310428505506775No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6980.11262024389220725No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG6940.11197485567505995No Hit
CTCTGGGATCGAGCAGAGCTTGGTGGAATGTGCTGGAGTTCCACTGCATG6860.11068407924076529No Hit
CCAAAAGACACCAAGTACTCTAGAACAGTTTCTCTTCCAAAAGTAAGACA6850.11052273218647847No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6760.10907060869789702No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA6710.10826387342646286No Hit
TTATTATCCTGAACATACAGTTAATCATTACTTCCAAACTAGACATTATT6650.10729579110074189No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6580.10616636172073406No Hit
ATGTTAACATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6540.10552097350358676No Hit
GTTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCC6470.10439154412357893No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCCGCAATGTCAACGACCGACCT6420.10358480885214477No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6420.10358480885214477No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6380.10293942063499747No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6280.10132595009212918No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTAGGTCTTCTGG6250.10084190892926867No Hit
CCTTTACCCCGTTGCTCGACAACGGTCAGGTCTTTGCCAAGTGTTTGCTG6210.10019652071212137No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)