FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442309
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATGCCAGCCTTCCACAGCGTATGTAAATAATGCCTGGTTT18670.42210309986909605No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC15780.35676416261030186No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC15550.35156417798416945No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT13980.3160686307536134No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTCAATCTCCTTTTTTCA13170.2977556414181036No Hit
CTCTTACAGTCAATGAAAAAAGGAGATTGAAATTAATTATGCCTGCTAGG12980.2934600019443421No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAAATC12940.2925556567919712No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTCAATGAAAAA12860.2907469664872295No Hit
GATTAACTGCATGTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGC12010.2715296319993489No Hit
GTATACATGCATATAACGGCATTAAGGCAGGATAGCCACATTGTGTAAAA11310.25570359183285896No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11310.25570359183285896No Hit
CTATTAATAATGTCCTCTTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG11240.25412098781620995No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT10820.24462536371631596No Hit
CTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCCTTTCCTCAC10190.23038192756647502No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10160.22970366870219688No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCGTTGACATACT10110.22857323726173331No Hit
CTCAAGAACAGTGTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA9580.2165906639928195No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA9580.2165906639928195No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGCGTATGTAAATAATGCCTGGT9550.21591240512854135No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT9040.20438200443581295No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA8540.1930776900311773No Hit
GGATATTAACATTGGGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA7960.1799646853217999No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7940.1795125127456145No Hit
CAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATG7930.17928642645752177No Hit
GGATAGAACCTAGCAGGCATAATTAATTTCAATCTCCTTTTTTCATTGAC7880.1781559950170582No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCATCACTTCCGGA7600.17182557895046224No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCTTGT7550.17069514750999867No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTGTCTCTTCCAAAAGTAAGA7460.16866037091716424No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA7270.1643647314434027No Hit
CTTACAGTCAATGAAAAAAGGAGATTGAAATTAATTATGCCTGCTAGGTT7260.16413864515530996No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTGCTGACTTCTTTCCTTCTATTCG7150.16165169598629014No Hit
AACATATTGTGCGGAAAATCAAGCAATGTTTTCGAAAACTGCCTGTAAAT7150.16165169598629014No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACCTACAAGGCC7130.1611995234101047No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCCTTTC6950.15712997022443587No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA6820.15419084847923056No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6660.15057346786974715No Hit
GCCTTAGAGTCTCCGCAACATTGTTCACCTCACCATACAGCACTCAGGCA6430.14537348324361474No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTCCGCACAATA6240.14107784376985322No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6230.1408517574817605No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC6230.1408517574817605No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT6200.14017349861748235No Hit
CTTTAATCCTGATTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAAG6190.13994741232938962No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCGTTATATGCATGTATA6110.13813872202464791No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6090.1376865494484625No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6060.13700829058418437No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6000.13565177285562807No Hit
ATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTAGGAT5970.13497351399134994No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC5890.13316482368660823No Hit
GTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC5830.13180830595805193No Hit
ATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5780.1306778745175884No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5630.12728658019619768No Hit
GTTCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCTGC5580.1261561487557341No Hit
CCTTATGAGTCCAAGGGATATTAACATTGGGATTCCCGAGATTGAGATCT5560.12570397617954868No Hit
TCCAGGGACTTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAAT5540.12525180360336327No Hit
ATGTTAATATGGGCCTAAAAATTAGACAACTACTGTGGTTTCACATTTCC5520.12479963102717782No Hit
GTTCATATATTGTTTACACAGAAAGGCCTTGTAGGTTGGCGAGAAAGTGA5480.12389528587480698No Hit
TAGCAGCAGGATGAAGAGGAATATGATAAAACGCCGCAGACACATCCAGC5460.12344311329862157No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG5460.12344311329862157No Hit
GCTTAGATTGTATACATGCATATAACGGCATTAAGGCAGGATAGCCACAT5450.12321702701052883No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5370.12140833670578711No Hit
ATATTATCCTGAACATGCAGTTAATCATTATTTCAAAACCAGGCATTATT5360.12118225041769441No Hit
CTCTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTC5220.11801704238439642No Hit
ATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTTTGCCAAG5210.1177909560963037No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5190.11733878352011828No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTGCTG5190.11733878352011828No Hit
CTCTAAGGCCTCCCGATACAGAGCTGAGGCGGTGTCGAGGAGATCTCGAA5140.11620835207965471No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAAATCATTAACCTAATCTCCTCC5140.11620835207965471No Hit
GTATCGGGAGGCCTTAGAGTCTCCGCAACATTGTTCACCTCACCATACAG5100.11530400692728388No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5070.11462574806300574No Hit
CTACTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGACACTGTTCT5030.11372140291063487No Hit
CTTGTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCAATCAGGATTAAAG5020.11349531662254217No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5000.11304314404635674No Hit
TATTAACATTGACATAGCTGACTACTAAGTCCCTGGATGCTTGGTCGTCC5000.11304314404635674No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTCCCTCGCAGGCGGCGATGGCC4890.11055619487733688No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGTTGTCT4880.11033010858924416No Hit
GTCCAAGGGATATTAACATTGGGATTCCCGAGATTGAGATCTTCTGCGAC4870.11010402230115146No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG4860.10987793601305874No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAAATCATT4830.1091996771487806No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTCCGC4830.1091996771487806No Hit
GCATATAACGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGC4820.10897359086068789No Hit
CATTTACAAGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT4760.1076170731321316No Hit
GTCTGAACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTT4730.10693881426785347No Hit
ATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTCCGCACAATATGTT4710.10648664169166805No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAGGTTGGCGA4700.10626055540357533No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG4700.10626055540357533No Hit
ATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTGAGG4690.10603446911548263No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC4650.10513012396311176No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTCCGCACAAT4650.10513012396311176No Hit
GATTGTATACATGCATATAACGGCATTAAGGCAGGATAGCCACATTGTGT4640.10490403767501905No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG4580.10354751994646276No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA4510.10196491592981377No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4510.10196491592981377No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4500.10173882964172105No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCT4470.10106057077744293No Hit
GTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG4450.10060839820125748No Hit
AGAACATATTGTGCGGAAAATCAAGCAATGTTTTCGAAAACTGCCTGTAA4440.10038231191316477No Hit
AGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA4430.10015622562507207No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)