FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384788
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT12310.3199164215100263No Hit
ACTATGCATTATTTACATACGCTGTGGAAGGCTGGCATTCTATATAAGAG10330.2684595153695022No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9910.25754441406696676No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA9720.2526066301443912No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG9580.24896826304354602No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA9580.24896826304354602No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8960.23285549445408898No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA8910.2315560776323586No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8840.229736894081936No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT8830.22947701071758994No Hit
CTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCCTTTCCTCAC8550.2222002765158997No Hit
ATTATTAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAA8380.21778225932201628No Hit
CATGTATACAATCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8360.21726249259332414No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT8230.21388400885682507No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT7870.20452820774036615No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA7830.2034886742829818No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7790.20244914082559748No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC7750.20140960736821314No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC7600.19751135690302193No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7500.1949125232595611No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG7410.1925735729804464No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7380.19179392288740812No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCGCTGACATACT7380.19179392288740812No Hit
ATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATATGGTT7090.18425730532137177No Hit
CTTATATAGAATGCCAGCCTTCCACAGCGTATGTAAATAATGCCTAGTTT6970.1811387049492188No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6910.1795794047631423No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTGTTAATAATATCCTCCTGT6710.17438173747622066No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCTCCGTG6520.1694439535536451No Hit
CCCTTAACTTCATGGGATATGTAATTGGATGTTGGGGTACTTTACCGCAA6420.1668451199101843No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC6210.1613875692589166No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT6120.15904861897980185No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6110.15878873561545578No Hit
CTTATATAGAATGCCAGCCTTCCACAGCGTATGTAAATAATGCATAGTTT5870.15255153487114984No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATATGGTTTAATGCC5630.14631433412684386No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCCTTTC5150.13383993263823196No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5100.13254051581650156No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5040.13098121563042506No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4970.12916203208000251No Hit
GTCCACAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4930.12812249862261818No Hit
GCTCCGAACGCAGGGTCCAACTGGTGATCGGGAAAGAATCCCAGAGGATT4920.12786261525827208No Hit
GTTAATAATATCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTGCC4910.127602731893926No Hit
CAATATGTTCTTGCGGTAAAGTACCCCAACATCCAATTACATATCCCATG4900.12734284852957992No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC4830.12552366497915735No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA4810.12500389825046518No Hit
GTTCCGAGCCAACTCAAACAATCCAGATTGGGACTTCAACCCCAACAAGG4730.12292483133569654No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4640.1205858810565818No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4590.11928646423485141No Hit
ATGATTAACTGCATGTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGG4580.11902658087050533No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4530.11772716404877491No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4530.11772716404877491No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4520.11746728068442883No Hit
CTTTAATCCTGATTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG4450.11564809713400626No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGCGTATGTAAATAATGCATAGT4410.11460856367662195No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4330.11252949676185328No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGCGTATGTAAATAATGCCTAGT4290.11148996330446895No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4270.11097019657577678No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4270.11097019657577678No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4270.11097019657577678No Hit
CATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4210.10941089638970031No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4200.10915101302535422No Hit
GTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC4060.10551264592450907No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA4030.10473299583147085No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4020.10447311246712476No Hit
CATTTACAGGAGGATATTATTAACAGATGTCAACAATATGTGGGCCCTCT4010.10421322910277868No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGGCGATGGCC3980.10343357900974043No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3920.10187427882366394No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)