FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384788
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT11000.28587170078068963No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9750.2533862802374295No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA9310.24195141220620187No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA9170.23831304510535672No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA9030.23467467800451156No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8910.2315560776323586No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA8550.2222002765158997No Hit
ACTATGCATTATTTACATACGCTGTGGAAGGCTGGCATTCTATATAAGAG8530.22168050978720752No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8380.21778225932201628No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8350.21700260922897802No Hit
ATTATTAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAA8320.2162229591359398No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT8220.21362412549247897No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT7890.20504797446905826No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT7840.20374855764732788No Hit
CTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCCTTTCCTCAC7820.20322879091863572No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG7710.2003700739108288No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT7660.19907065708909844No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC7570.19673170680998367No Hit
ATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATATGGTT7450.1936131064378307No Hit
CATGTATACAATCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7300.18971485597263948No Hit
CCCTTAACTTCATGGGATATGTAATTGGATGTTGGGGTACTTTACCGCAA7270.18893520587960122No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7160.18607648887179434No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC7140.18555672214310218No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCGCTGACATACT7020.1824381217709492No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6940.18035905485618053No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTGTTAATAATATCCTCCTGT6560.17048348701102944No Hit
CTTATATAGAATGCCAGCCTTCCACAGCGTATGTAAATAATGCCTAGTTT6510.16918407018929904No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT6450.16762477000322257No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6120.15904861897980185No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC6100.15852885225110971No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCTCCGTG5890.153071301599842No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATATGGTTTAATGCC5770.149952701227689No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5680.1476137509485743No Hit
GCTCCGAACGCAGGGTCCAACTGGTGATCGGGAAAGAATCCCAGAGGATT5530.14371550048338308No Hit
CTTATATAGAATGCCAGCCTTCCACAGCGTATGTAAATAATGCATAGTTT5340.1387777165608075No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA5290.1374782997390771No Hit
GTTCCGAGCCAACTCAAACAATCCAGATTGGGACTTCAACCCCAACAAGG5190.13487946609561627No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCCTTTC5170.1343596993669241No Hit
GTTAATAATATCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTGCC5130.1333201659095398No Hit
GTCCACAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5100.13254051581650156No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC4730.12292483133569654No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4700.1221451812426583No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4700.1221451812426583No Hit
ATGATTAACTGCATGTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGG4590.11928646423485141No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4540.117987047413121No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4530.11772716404877491No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGCGTATGTAAATAATGCCTAGT4520.11746728068442883No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4460.11590798049835234No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4430.11512833040531409No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4410.11460856367662195No Hit
GCCTCGTCGAGGTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCC4290.11148996330446895No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4280.11123007994012286No Hit
CAATATGTTCTTGCGGTAAAGTACCCCAACATCCAATTACATATCCCATG4270.11097019657577678No Hit
GTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC4230.10993066311839246No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGCGTATGTAAATAATGCATAGT4230.10993066311839246No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4220.10967077975404639No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4090.10629229601754732No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4060.10551264592450907No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4000.1039533457384326No Hit
TCTTTCTGTTCCCAATCCTCTGGGATTCTTTCCCGATCACCAGTTGGACC3990.10369346237408651No Hit
GGATATTATTAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG3940.10239404555235611No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA3930.10213416218801002No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)