FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283478
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCCTAT8190.2889113088140879No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7880.27797571592857295No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG7630.2691566894079964No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7010.24728550363696655No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6540.23070573377828263No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5380.1897854507228074No Hit
CATGTATACAATCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5350.18872716754033825No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA5330.1880216454186921No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5180.18273022950634618No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAATTCCCTCCTTTCCTCAC5170.1823774684455231No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5020.17708605253317716No Hit
CTATATAAGAGAGAAACTACACGCAGTGCCTCATTTTGTGGGTCACCATA5000.17638053041153107No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA4910.1732056808641235No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGAACTTTACCGCAG4790.16897254813424675No Hit
ATTCAAGATCTCCGCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGA4680.16509217646519306No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4640.1636811322219008No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4460.1573314331270857No Hit
CTATAACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA4360.15380382251885508No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT4300.1516872561539167No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4270.1506289729714475No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT4260.15027621191062446No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4240.14957068978897833No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAATGAAAAA4120.1453375570591016No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAATTTG4070.1435737517549863No Hit
GGATAATAGGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT4010.1414571853900479No Hit
ATATAAGAGAGAAACTACACGCAGTGCCTCATTTTGTGGGTCACCATATT3840.13546024735605586No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC3720.13122711462617911No Hit
TTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT3490.12311361022724868No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG3460.12205532704477949No Hit
GTACAATATGTTCCTGCGGTAAAGTTCCCCAACTTCCAATTACATATCCC3450.12170256598395643No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC3400.1199387606798411No Hit
CTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT3250.11464734476749519No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3200.11288353946337987No Hit
CTCTTACAATTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG3130.11041421203761843No Hit
CTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGA3100.10935592885514926No Hit
ATTAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAATGAAAAAAG2990.10547555718609557No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA2930.10335899082115721No Hit
GAGTTCAGCAGTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGC2860.10088966339539578No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA2840.10018414127374964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)