FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283478
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG8780.30972421140264855No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7890.278328476989396No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCCTAT7220.25469348591425084No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6730.23740819393392082No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6530.23035297271745955No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA5750.20283760997326072No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA5230.18449403481046148No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5210.18378851268881535No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5200.18343575162799228No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5010.17673329147235411No Hit
CTATATAAGAGAGAAACTACACGCAGTGCCTCATTTTGTGGGTCACCATA4990.176027769350708No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAATTCCCTCCTTTCCTCAC4910.1732056808641235No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4600.16227008797860856No Hit
CTATAACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA4590.16191732691778551No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAATTTG4520.15944799949202407No Hit
ATTCAAGATCTCCGCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGA4490.1583897163095549No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT4220.1488651676673322No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4210.14851240660650916No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGAACTTTACCGCAG4180.14745412342403996No Hit
CATGTATACAATCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4160.14674860130239384No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAATGAAAAA4130.14569031811992464No Hit
GGATAATAGGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT3910.13792957478181728No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA3890.13722405266017115No Hit
ATATAAGAGAGAAACTACACGCAGTGCCTCATTTTGTGGGTCACCATATT3820.1347547252344097No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC3730.13157987568700216No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT3720.13122711462617911No Hit
GTACAATATGTTCCTGCGGTAAAGTTCCCCAACTTCCAATTACATATCCC3680.12981607038288684No Hit
TTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT3670.1294633093220638No Hit
GAGTTCAGCAGTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGC3560.12558293765301012No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC3560.12558293765301012No Hit
CTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGA3490.12311361022724868No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG3460.12205532704477949No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3310.11676391113243356No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA3310.11676391113243356No Hit
ATATTGTACTGAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA3230.11394182264584905No Hit
CTCTTACAATTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG3170.1118252562809107No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG3160.11147249522008762No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3150.11111973415926456No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAGGGTTTAATGCC3050.10759212355103394No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA3050.10759212355103394No Hit
GTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG2990.10547555718609557No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA2980.1051227961252725No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG2980.1051227961252725No Hit
CTTACAATTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT2870.10124242445621882No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT2840.10018414127374964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)