FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455964
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26360.578115816160925No Hit
TCCAAAGAGTGTGCAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA16460.3609934117605776No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGCAAATAATGTCTCGT16390.35945820284057517No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA11830.25945030748041514No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT10740.2355449114403769No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10720.2351062803203762No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9620.21098156872033755No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAATATT9560.20966567536033548No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA9050.19848058180031758No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA8600.18861138160030178No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG8500.18641822600029828No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAGTTATGCCTGCTAGG8110.17786491916028457No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGA8030.17611039468028178No Hit
GGAACATATTGTACAAAAAATCAAACTGTGCTTTAGAAAACTTCCTGTAA7810.17128545236027407No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC7460.16360940776026178No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT7400.1622935144002597No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7000.15352089200024563No Hit
CAATATTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTATTATC6990.15330157644024528No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT6860.1504504741602407No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6630.14540621628023265No Hit
GTTTACAGGAAGTTTTCTAAAGCACAGTTTGATTTTTTGTACAATATGTT6620.1451869007202323No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGAAAGCACGACTGAAT6400.14036195840022456No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC6310.1383881183602214No Hit
CTTCAGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGAGGGAGTTCT6200.13597564720021757No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAGCACAGTTTGATTTTTTGT6160.13509838496021617No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT6080.13334386048021335No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGCAAATAATGTCTCGTCTGGA6040.13246659824021195No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6010.1318086515602109No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5950.13049275820020878No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGCAAATAATGTCTC5930.13005412708020808No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC5920.12983481152020773No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG5840.1280802870402049No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATTTAGCCACCTGGGTGGGAAGTAA5790.1269837092402032No Hit
TGATTAACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGG5790.1269837092402032No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG5720.12544850032020072No Hit
GCACTGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG5630.12347466028019756No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5630.12347466028019756No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5620.1232553447201972No Hit
GGAGGATATTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAA5450.11952698020019124No Hit
GTTGTGAGTATGCCCTGAGCTTGAGGGCTCCACCCCAACAGTCCCCCATG5450.11952698020019124No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5430.11908834908019054No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG5350.11733382460018774No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA5320.11667587792018669No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5320.11667587792018669No Hit
GGATATTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATG5240.11492135344018388No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC5180.11360546008018177No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC5060.11097367336017755No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC5010.1098770955601758No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA4940.10834188664017334No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA4830.10592941548016949No Hit
ACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA4820.10571009992016912No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA4750.10417489100016668No Hit
TAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTAC4710.10329762876016527No Hit
CTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGGA4650.10198173540016317No Hit
TCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCCAG4650.10198173540016317No Hit
GGTCTGGTCTGTGCCAAGTGTTTGCTGATGCAACCCCCACTGGTTGGGGC4650.10198173540016317No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4630.10154310428016247No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA4600.10088515760016141No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4590.10066584204016106No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)