FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455964
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26240.5754840294409208No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGCAAATAATGTCTCGT17730.38884648788062215No Hit
TCCAAAGAGTGTGCAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA16500.361870674000579No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA12440.27282855664043654No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11240.2465106894403944No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAATATT9880.2166837732803467No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT9140.20045442184032075No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9140.20045442184032075No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA9130.2002351062803204No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG8600.18861138160030178No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT8030.17611039468028178No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAGTTATGCCTGCTAGG7610.16689914116026705No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7460.16360940776026178No Hit
GGAACATATTGTACAAAAAATCAAACTGTGCTTTAGAAAACTTCCTGTAA7260.15922309656025477No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA7260.15922309656025477No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGCAAATAATGTCTC7120.15615267872024985No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT7090.15549473204024877No Hit
GTTTACAGGAAGTTTTCTAAAGCACAGTTTGATTTTTTGTACAATATGTT6770.14847663412023757No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGCAAATAATGTCTCGTCTGGA6650.14584484740023335No Hit
CTTCAGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGAGGGAGTTCT6510.14277442956022843No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGA6470.14189716732022703No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6340.1390460650402225No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6310.1383881183602214No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC6130.1344404382802151No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6000.13158933600021055No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC5930.13005412708020808No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG5920.12983481152020773No Hit
CAATATTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTATTATC5890.12917686484020668No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5850.1282996026002053No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG5770.12654507812020246No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGAAAGCACGACTGAAT5700.12500986920020002No Hit
GTTGTGAGTATGCCCTGAGCTTGAGGGCTCCACCCCAACAGTCCCCCATG5660.12413260696019861No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC5640.12369397584019791No Hit
GGAGGATATTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAA5550.12172013580019475No Hit
GCACTGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG5550.12172013580019475No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAGCACAGTTTGATTTTTTGT5550.12172013580019475No Hit
GTTTGATTTTTTGTACAATATGTTCCTGTGGCAATGTGCCCCAACTCCCA5530.12128150468019405No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG5450.11952698020019124No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA5450.11952698020019124No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC5350.11733382460018774No Hit
GGTCTGGTCTGTGCCAAGTGTTTGCTGATGCAACCCCCACTGGTTGGGGC5330.11689519348018704No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA5270.11557930012018491No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATTTAGCCACCTGGGTGGGAAGTAA5230.11470203788018353No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG5210.11426340676018282No Hit
GGATATTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATG5160.11316682896018106No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5080.11141230448017826No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT5070.1111929889201779No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5060.11097367336017755No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA5060.11097367336017755No Hit
TGATTAACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGG5030.11031572668017652No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA5010.1098770955601758No Hit
CTATTCTGTGTTGGGGTGAGTTAATGAATTTAGCCACCTGGGTGGGAAGT4920.10790325552017266No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4900.10746462440017195No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4850.10636804660017019No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC4800.10527146880016844No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA4790.10505215324016809No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4710.10329762876016527No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4700.10307831320016492No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4600.10088515760016141No Hit
CTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGGA4590.10066584204016106No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4570.10022721092016038No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)