FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459345
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24340.5298849448671478No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG17290.3764055339668441No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG12740.2773514460808325No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT12560.2734328228238035No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA10240.22292612306654042No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC10020.2181366946412827No Hit
ATTAACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT9820.21378266880013935No Hit
GTGTAAAAGGTGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9820.21378266880013935No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG9730.21182335717162484No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC8700.18940012408973647No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT8630.18787621504533628No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8220.17895046207099238No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT8190.17829735819482087No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8080.17590264398219202No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7740.16850080005224832No Hit
ATCCCAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT7630.16610608583961947No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7290.15870424190967575No Hit
GTTGTTAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCGCCGTG7020.1528263070241322No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6970.15173780056384634No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC6890.149996190227389No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA6870.14956078764327466No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG6680.14542446309418847No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6680.14542446309418847No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6660.14498906051007412No Hit
GTTCTATCCCAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC6530.14215894371333093No Hit
CATTTGAACCCCAATAAAACTAAACGTTGGGGCTATTCCCTTAACTTCAT6490.14128813854510225No Hit
TTGACATACTTTCCAATCAATAGGTCGATTTACAGGCAGTTTTCGAAAAC6490.14128813854510225No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6450.1404173333768736No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6420.13976422950070208No Hit
CATGTATACAATCAAAGAAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6370.13867572304041625No Hit
GTCTAATTTTTAGGCCCATATTAACATTGACATAGCTGACTACTAATTCC6350.1382403204563019No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTTGTTGACATCGCTGAGAGTCCAA6350.1382403204563019No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6290.1369341127039589No Hit
ATATTGTACTTAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATCGA5890.12822606102167217No Hit
CAATAAAACTAAACGTTGGGGCTATTCCCTTAACTTCATGGGATATGTAA5750.1251782429328718No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5650.12300123001230012No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5650.12300123001230012No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5630.12256582742818578No Hit
ACCCATCTCGGGGCCGTTTGGGCCTCTACCGTCCCCTTCTTCATCTGCCG5590.12169502225995713No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCA5580.12147732096789994No Hit
AAATCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGA5360.11668789254264224No Hit
GCCTAATCATCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5350.11647019125058508No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA5270.11472858091412773No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5220.11364007445384189No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT5180.1127692692856132No Hit
GAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCA5130.11168076282532736No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5090.1108099576570987No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5010.10906834732064136No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC4990.108632944736527No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGTGCAGCA4950.10776213956829833No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4950.10776213956829833No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCCAATAAA4920.10710903569212683No Hit
CCTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTAT4910.10689133440006968No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4910.10689133440006968No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAA4900.10667363310801249No Hit
GTTTTATTGGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT4860.10580282793978381No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4840.10536742535566948No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTTAAAACTAGGCATTATT4820.10493202277155515No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4790.10427891889538364No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4740.1031904124350978No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTCC4600.10014259434629744No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)